Key Product Details

Species Reactivity

Validated:

Bovine

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

LYOPH mg/ml

View all Acetylcholinesterase/ACHE Antibodies »

Product Summary for Acetylcholinesterase/ACHE Antibody

Immunogen

Acetylcholinesterase/ACHE [Bovine Erythrocytes] (Uniprot: P23795)

Reactivity Notes

Cross reactivity against Acetylcholinesterase/ACHE from other sources is unknown.

Localization

Cell Membrane and Secreted

Specificity

Cross reactivity against Acetylcholinesterase/ACHE from other sources is unknown.

Marker

Early Neuronal Development Marker

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Acetyl Cholinesterase [Bovine Erythrocytes]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Acetylcholinesterase/ACHE Antibody

Western Blot: Acetylcholinesterase/ACHE Antibody [NB600-911]

Western Blot: Acetylcholinesterase/ACHE Antibody [NB600-911]

Western Blot: Acetylcholinesterase/ACHE Antibody [NB600-911] - Analysis using the Biotin conjugate of NB600-911. Detection of antibody with pyranose oxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1: 3000 dilution of primary antibody. Detection shown was using Dylight 488 conjugated Donkey anti goat 1:10K in TBS 1 hr RT.

Applications for Acetylcholinesterase/ACHE Antibody

Application
Recommended Usage

ELISA

1:5000-1:20000

Immunoprecipitation

1:100

Western Blot

1:500-1:5000
Application Notes
This product has been tested by western blot and is suitable to be assayed against 1.0 ug of Acetyl Cholinesterase [Bovine Erythrocytes] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,200 to 1:5,000 of the reconstitution concentration is suggested for this product.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: Acetylcholinesterase/ACHE

Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.

Alternate Names

ACHE, ARACHE, N-ACHE

Gene Symbol

ACHE

UniProt

P23795 (Bovine)

Additional Acetylcholinesterase/ACHE Products

Product Documents for Acetylcholinesterase/ACHE Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Acetylcholinesterase/ACHE Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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