Acidic Calponin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38643

Novus Biologicals, part of Bio-Techne
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NBP2-38643
NBP2-38643-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Acidic Calponin Antibodies »

Product Summary for Acidic Calponin Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PKYCAAPTEPVIHNGSQGTGTNGSEISDSDYQAEYPDEYHGEYQDDYPRDYQYSDQGIDY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Acidic Calponin Antibody

Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP2-38643]

Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP2-38643]

Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP2-38643] - Analysis in human placenta and skeletal muscle tissues. Corresponding Acidic Calponin RNA-seq data are presented for the same tissues.
Acidic Calponin Antibody

Western Blot: Acidic Calponin Antibody [NBP2-38643] -

Western Blot: Acidic Calponin Antibody [NBP2-38643] - Analysis in human cell lines U2OS and MCF-7 using Anti-CNN3 antibody. Corresponding CNN3 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
Immunocytochemistry/ Immunofluorescence: Acidic Calponin Antibody [NBP2-38643]

Immunocytochemistry/ Immunofluorescence: Acidic Calponin Antibody [NBP2-38643]

Immunocytochemistry/Immunofluorescence: Acidic Calponin Antibody [NBP2-38643] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & actin filaments.
View 5 more images

Applications for Acidic Calponin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Acidic Calponin

Acidic Calponin, or CNN3 for short, consists of a 329 amino acid isoform that is 36 kDa, and is involved in the regulation of smooth muscle contraction. Current research is being conducted on the relationship between acidic calponin and several diseases and disorders, including aortic aneurysm and neuronitis. The protein is not associated with any pathways, but it does interact with the proteins FYN, SLX4, ATG5, LCP1, and EMD.

Alternate Names

calponin 3, acidic, Calponin, acidic isoform, calponin-3, dJ639P13.2.2 (acidic calponin 3)

Gene Symbol

CNN3

UniProt

Q15417 (Human)

Additional Acidic Calponin Products

Product Documents for Acidic Calponin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Acidic Calponin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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