Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1G1

Concentration

0.81 mg/ml

View all ACSM5 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ACSM5(NP_060358) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

64.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACSM5 Antibody (OTI1G1)

Western Blot: ACSM5 Antibody (OTI1G1) [NBP2-01874]

Western Blot: ACSM5 Antibody (OTI1G1) [NBP2-01874]

Western Blot: ACSM5 Antibody (1G1) [NBP2-01874] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACSM5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACSM5.
Immunocytochemistry/Immunofluorescence: ACSM5 Antibody (1G1) [NBP2-01874] Staining of COS7 cells transiently transfected by pCMV6-ENTRY ACSM5.

Applications for ACSM5 Antibody (OTI1G1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.81 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ACSM5

ACSM5 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C(4) to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids (in vitro)

Alternate Names

acyl-CoA synthetase medium-chain family member 5, acyl-coenzyme A synthetase ACSM5, mitochondrial, EC 6.2.1, EC 6.2.1.2, FLJ20581, MACS3

Entrez Gene IDs

54988 (Human)

Gene Symbol

ACSM5

Additional ACSM5 Products

Product Documents for ACSM5 Antibody (OTI1G1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ACSM5 Antibody (OTI1G1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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