Actin Alpha 1 Cardiac Muscle Antibody (33-32-R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32032
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # 33-32-R
Concentration
1 mg/ml
Product Summary for Actin Alpha 1 Cardiac Muscle Antibody (33-32-R)
Immunogen
Synthetic peptide within human Actin Alpha 1 Cardiac Muscle aa 1-50 / 377. (Uniprot: P68032)
Localization
Cytoplasm, cytoskeleton.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Actin Alpha 1 Cardiac Muscle Antibody (33-32-R)
Western Blot: Actin Alpha 1 Cardiac Muscle Antibody (33-32-R) [NBP3-32032]
Western Blot: Actin Alpha 1 Cardiac Muscle Antibody (33-32-R) [NBP3-32032] - Western blot analysis of Actin Alpha 1 Cardiac Muscle on different lysates with Mouse anti-Actin Alpha 1 Cardiac Muscle antibody (NBP3-32032) at 1/1,000 dilution.Lane 1: Mouse lung tissue lysate
Lane 2: Mouse heart tissue lysate
Lane 3: Rat heart tissue lysate
Lysates/proteins at 40 ug/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32032) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry: Actin Alpha 1 Cardiac Muscle Antibody (33-32-R) [NBP3-32032]
Immunohistochemistry: Actin Alpha 1 Cardiac Muscle Antibody (33-32-R) [NBP3-32032] - Immunofluorescence analysis of paraffin-embedded human heart tissue labeling Actin Alpha 1 Cardiac Muscle with Mouse anti-Actin Alpha 1 Cardiac Muscle antibody (NBP3-32032) at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32032, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: Actin Alpha 1 Cardiac Muscle Antibody (33-32-R) [NBP3-32032]
Immunohistochemistry: Actin Alpha 1 Cardiac Muscle Antibody (33-32-R) [NBP3-32032] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Mouse anti-Actin Alpha 1 Cardiac Muscle antibody (NBP3-32032) at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32032) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Applications for Actin Alpha 1 Cardiac Muscle Antibody (33-32-R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:1000
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: alpha-Cardiac Actin
The Actin protein, a component of the eukaryotic cytoskeleton, is highly conserved between multiple species. Actin is a globular protein of approximately 42kDa. Levels of the Actin protein may vary between different cell types, but are consistent within a given cell type. For this reason, Actin protein levels are often considered a 'house-keeper', an internal control for protein loading.
The actin network represents an essential component of the cell cytoskeleton, which is made up of microfilaments composed of linear assemblies of actin molecules. Actin assembly is known to be important for the remodeling of cell shape during cell motility, cell division and morphogenesis. The biological functions of actin occur in concert with a large number of Actin binding proteins, including cofilin, actin depolymerization factor (ADF), myosins, and many more.
Actin molecules accumulate as Hirano bodies, in neurons of patients with Alzheimer's disease, Creutzfeldt-Jakob disease, and other neurodegenerative diseases. Hirano bodies are intracellular aggregates of actin and actin-associated proteins.
Long Name
Actin, Alpha, Cardiac Muscle 1
Alternate Names
ACTC, ACTC1, ASD5, Cardiac alpha-Actin, CMD1R, CMH11, LVNC4
Gene Symbol
ACTC1
Additional alpha-Cardiac Actin Products
Product Documents for Actin Alpha 1 Cardiac Muscle Antibody (33-32-R)
Product Specific Notices for Actin Alpha 1 Cardiac Muscle Antibody (33-32-R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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