Actin Alpha 1 Cardiac Muscle Antibody (RM257)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61474
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM257
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to the N-terminus of Human Actin Alpha 1 Cardiac Muscle
Reactivity Notes
This antibody may also react to bovine or rat Actin Alpha 1 Cardiac Muscle, as predicted by immunogen homology.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Actin Alpha 1 Cardiac Muscle Antibody (RM257)
Western Blot: Actin Alpha 1 Cardiac Muscle Antibody (RM257) [NBP2-61474] -
Western Blot: Actin Alpha 1 Cardiac Muscle Antibody (RM257) [NBP2-61474] - Western Blot of Mouse heart tissue lysate, using NBP2-61474 at a 1:1000 dilution
Immunohistochemistry: Actin Alpha 1 Cardiac Muscle Antibody (RM257) [NBP2-61474] -
Immunohistochemistry: Actin Alpha 1 Cardiac Muscle Antibody (RM257) [NBP2-61474] - Immunohistochemical staining of FFPE Human heart tissue sections using NBP2-61474 at a 1:1000 dilutionApplications for Actin Alpha 1 Cardiac Muscle Antibody (RM257)
Application
Recommended Usage
Immunohistochemistry
1:1000 - 1:2000
Immunohistochemistry-Frozen
1:10 - 1:500
Immunohistochemistry-Paraffin
1:10 - 1:500
Western Blot
1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using NBP2-61474 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: alpha-Cardiac Actin
The Actin protein, a component of the eukaryotic cytoskeleton, is highly conserved between multiple species. Actin is a globular protein of approximately 42kDa. Levels of the Actin protein may vary between different cell types, but are consistent within a given cell type. For this reason, Actin protein levels are often considered a 'house-keeper', an internal control for protein loading.
The actin network represents an essential component of the cell cytoskeleton, which is made up of microfilaments composed of linear assemblies of actin molecules. Actin assembly is known to be important for the remodeling of cell shape during cell motility, cell division and morphogenesis. The biological functions of actin occur in concert with a large number of Actin binding proteins, including cofilin, actin depolymerization factor (ADF), myosins, and many more.
Actin molecules accumulate as Hirano bodies, in neurons of patients with Alzheimer's disease, Creutzfeldt-Jakob disease, and other neurodegenerative diseases. Hirano bodies are intracellular aggregates of actin and actin-associated proteins.
Long Name
Actin, Alpha, Cardiac Muscle 1
Alternate Names
ACTC, ACTC1, ASD5, Cardiac alpha-Actin, CMD1R, CMH11, LVNC4
Gene Symbol
ACTC1
Additional alpha-Cardiac Actin Products
Product Documents for Actin Alpha 1 Cardiac Muscle Antibody (RM257)
Product Specific Notices for Actin Alpha 1 Cardiac Muscle Antibody (RM257)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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