Actin Gamma 1 Antibody (1G6)

Novus Biologicals, part of Bio-Techne | Catalog # H00000071-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Proximity Ligation Assay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1G6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Actin Gamma 1 Antibodies »

Product Specifications

Immunogen

ACTG1 (AAH53572, 1 a.a. ~ 375 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Specificity

ACTG1 - actin, gamma 1 (1G6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Actin Gamma 1 Antibody (1G6)

Proximity Ligation Assay: Actin Gamma 1 Antibody (1G6) [H00000071-M01]

Proximity Ligation Assay: Actin Gamma 1 Antibody (1G6) [H00000071-M01]

Proximity Ligation Assay: Actin Gamma 1 Antibody (1G6) [H00000071-M01] - Analysis of protein-protein interactions between VASP and ACTG1. HeLa cells were stained with anti-VASP rabbit purified polyclonal 1:1200 and anti-ACTG1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
ELISA: Actin Gamma 1 Antibody (1G6) [H00000071-M01]

ELISA: Actin Gamma 1 Antibody (1G6) [H00000071-M01]

ELISA: Actin Gamma 1 Antibody (1G6) [H00000071-M01] - Detection limit for recombinant GST tagged ACTG1 is 1 ng/ml as a capture antibody.

Applications for Actin Gamma 1 Antibody (1G6)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Proximity Ligation Assay

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Actin Gamma 1

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells.

Alternate Names

ACT, ACTB, ACTG, actin, cytoplasmic 2, actin, gamma 1, cytoskeletal gamma-actin, deafness, autosomal dominant 20; deafness, autosomal dominant 26, DFNA20, DFNA26, Gamma-actin

Entrez Gene IDs

71 (Human)

Gene Symbol

ACTG1

UniProt

AAH53572 (Human)

Additional Actin Gamma 1 Products

Product Documents for Actin Gamma 1 Antibody (1G6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Actin Gamma 1 Antibody (1G6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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