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Actin Gamma 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15258

Novus Biologicals, part of Bio-Techne
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NBP2-15258

Key Product Details

Species Reactivity

Validated:

Mouse, Feline

Predicted:

Bovine (100%), Canine (100%), Chicken (99%), Human (100%), Primate (100%), Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Actin Gamma 1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (100%), Chimpanzee (100%).

Localization

Cytoplasm; cytoskeleton

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Actin Gamma 1 Antibody

Western Blot: Actin Gamma 1 Antibody [NBP2-15258]

Western Blot: Actin Gamma 1 Antibody [NBP2-15258]

Western Blot: Actin Gamma 1 Antibody [NBP2-15258] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE, antibody diluted at 1:500.
Actin Gamma 1 Antibody

Western Blot: Actin Gamma 1 Antibody [NBP2-15258] -

Western Blot: Actin Gamma 1 Antibody [NBP2-15258] - PG-4 whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Actin Gamma 1 antibody (NBP2-15258) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for Actin Gamma 1 Antibody

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Actin Gamma 1

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells. [provided by RefSeq]

Alternate Names

ACT, ACTB, ACTG, actin, cytoplasmic 2, actin, gamma 1, cytoskeletal gamma-actin, deafness, autosomal dominant 20; deafness, autosomal dominant 26, DFNA20, DFNA26, Gamma-actin

Gene Symbol

ACTG1

UniProt

Additional Actin Gamma 1 Products

Product Documents for Actin Gamma 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Actin Gamma 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.