Actin (Muscle Specific) Antibody (HHF35) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-09165
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Canine, Chicken, Feline, Primate, Rabbit
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # HHF35
Format
BSA Free
Concentration
1 mg/ml
Product Summary for Actin (Muscle Specific) Antibody (HHF35) - BSA Free
Immunogen
SDS extracted protein fraction of human myocardium
Specificity
The mouse monoclonal antibody HHF35 recognizes muscle-specific alpha and gamma actin (42 kDa) in various species. This antibody stains skeletal, smooth and myocardial cells as well as myoepithelial cells and pericytes of small vessels. It is a widely used marker of muscle and muscle-derived cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Actin (Muscle Specific) Antibody (HHF35) - BSA Free
![Western Blot: Actin (Muscle Specific) Antibody (HHF35)BSA Free [NBP3-09165]](https://resources.bio-techne.com/images/products/Actin-Muscle-Specific-Antibody-HHF35-Western-Blot-NBP3-09165-img0001.jpg "Western Blot: Actin (Muscle Specific) Antibody (HHF35)BSA Free [NBP3-09165]")
Western Blot: Actin (Muscle Specific) Antibody (HHF35)BSA Free [NBP3-09165]
Western Blot: Actin (Muscle Specific) Antibody (HHF35) [NBP3-09165] - Analysis of muscle-specific actin using the monoclonal antibody HHF35. Lane 1: murine femoral muscle Lane 2: murine heart Lane 3: HeLa Lane 4: HUVEC![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) - BSA Free [NBP3-09165]](https://resources.bio-techne.com/images/products/Actin-Muscle-Specific-Antibody-HHF35-Immunohistochemistry-Paraffin-NBP3-09165-img0002.jpg "Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) - BSA Free [NBP3-09165]")
Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) - BSA Free [NBP3-09165]
Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) [NBP3-09165] - Staining of human muscle (paraffin-embedded sections) with anti-muscle-specific actin (HHF35).Applications for Actin (Muscle Specific) Antibody (HHF35) - BSA Free
Application
Recommended Usage
Western Blot
1 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Actin (Muscle Specific)
Alternate Names
ACTA, ACTC, actin, alpha 2, smooth muscle, aorta, actin, alpha cardiac muscle 1, actin, alpha skeletal muscle, actin, aortic smooth muscle, ACTSA, alpha-cardiac actin, ASD5, ASMA, Cell growth-inhibiting gene 46 protein, CFTD, CFTD1, CFTDM, CMD1R, CMH11, MPFD, NEM1, NEM2, NEM3, nemaline myopathy type 3
Gene Symbol
ACTA1
Additional Actin (Muscle Specific) Products
Product Documents for Actin (Muscle Specific) Antibody (HHF35) - BSA Free
Product Specific Notices for Actin (Muscle Specific) Antibody (HHF35) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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