actin-related protein 2/3 complex subunit 1B [p Thr21] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23382
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Chicken, Xenopus
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for actin-related protein 2/3 complex subunit 1B [p Thr21] Antibody
Immunogen
Phospho-actin-related protein 2/3 complex subunit 1B (Thr-21) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding threonine 21 in human Arpc1b. This peptide sequence is highly conserved in rat, mouse, chicken, and fish Arpc1b, and has some homology to the conserved site in isoform Arpc1a. [UniProt# O15143]
Modification
p Thr21
Specificity
The antibody detects a 41 kDa protein corresponding to the molecular mass of actin-related protein 2/3 complex subunit 1B on SDS-PAGE immunoblots of human A431 and mouse C2C12 treated with calyculin A. This reactivity is removed with lambda phosphatase treatment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for actin-related protein 2/3 complex subunit 1B [p Thr21] Antibody
![actin-related protein 2/3 complex subunit 1B [p Thr21] Antibody](https://resources.bio-techne.com/images/products/nbp3-23382_rabbit-actin-related-protein-2-3-complex-subunit-1b-p-thr21-pab-199202312523618.jpg "Western Blot: actin-related protein 2/3 complex subunit 1B [p Thr21] Antibody [NBP3-23382] -")
Western Blot: actin-related protein 2/3 complex subunit 1B [p Thr21] Antibody [NBP3-23382] -
Western Blot: actin-related protein 2/3 complex subunit 1B [p Thr21] Antibody [NBP3-23382] - Western blot analysis of Arpc1b phosphorylation in human A431 stimulated with calyculin A (100 nM) for 30 min (lanes 1 & 3). The blots were treated with lambda phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Arpc1b (C-terminal region) (lanes 1 & 2) or anti-Arpc1b (Thr-21) (lanes 3 & 4).Applications for actin-related protein 2/3 complex subunit 1B [p Thr21] Antibody
Application
Recommended Usage
Western Blot
1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: actin-related protein 2/3 complex subunit 1B
Alternate Names
actin related protein 2/3 complex, subunit 1B (41 kD), actin related protein 2/3 complex, subunit 1B, 41kDa, actin-related protein 2/3 complex subunit 1B, ARC41p41-ARCp40-ARC, Arp2/3 complex 41 kDa subunit, ARP2/3 protein complex subunit p41
Gene Symbol
ARPC1B
Additional actin-related protein 2/3 complex subunit 1B Products
Product Documents for actin-related protein 2/3 complex subunit 1B [p Thr21] Antibody
Product Specific Notices for actin-related protein 2/3 complex subunit 1B [p Thr21] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...