ACTR3 Antibody (8K4F2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16424
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 8K4F2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 319-418 of human ACTR3 (P61158). IVLSGGSTMFRDFGRRLQRDLKRTVDARLKLSEELSGGRLKPKPIDVQVITHHMQRYAVWFGGSMLASTPEFYQVCHTKKDYEEIGPSICRHNPVFGVMS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ACTR3 Antibody (8K4F2)
Western Blot: ACTR3 Antibody (8K4F2) [NBP3-16424]
Western Blot: ACTR3 Antibody (8K4F2) [NBP3-16424] - Western blot analysis of extracts of various cell lines, using ACTR3 Rabbit mAb (NBP3-16424) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.Immunocytochemistry/ Immunofluorescence: ACTR3 Antibody (8K4F2) [NBP3-16424]
Immunocytochemistry/Immunofluorescence: ACTR3 Antibody (8K4F2) [NBP3-16424] - Immunofluorescence analysis of U-2 OS cells using ACTR3 Rabbit mAb (NBP3-16424) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: ACTR3 Antibody (8K4F2) [NBP3-16424]
Immunohistochemistry-Paraffin: ACTR3 Antibody (8K4F2) [NBP3-16424] - Immunohistochemistry of paraffin-embedded mouse spleen using ACTR3 Rabbit mAb (NBP3-16424) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for ACTR3 Antibody (8K4F2)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ACTR3
Long Name
Actin-related protein 3
Alternate Names
ARP3
Gene Symbol
ACTR3
Additional ACTR3 Products
Product Documents for ACTR3 Antibody (8K4F2)
Product Specific Notices for ACTR3 Antibody (8K4F2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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