ACTR3 Antibody (8K4F2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16424

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16424-100ul
NBP3-16424-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8K4F2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ACTR3 Antibodies »

Product Summary for ACTR3 Antibody (8K4F2)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 319-418 of human ACTR3 (P61158). IVLSGGSTMFRDFGRRLQRDLKRTVDARLKLSEELSGGRLKPKPIDVQVITHHMQRYAVWFGGSMLASTPEFYQVCHTKKDYEEIGPSICRHNPVFGVMS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ACTR3 Antibody (8K4F2)

Western Blot: ACTR3 Antibody (8K4F2) [NBP3-16424]

Western Blot: ACTR3 Antibody (8K4F2) [NBP3-16424]

Western Blot: ACTR3 Antibody (8K4F2) [NBP3-16424] - Western blot analysis of extracts of various cell lines, using ACTR3 Rabbit mAb (NBP3-16424) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunocytochemistry/ Immunofluorescence: ACTR3 Antibody (8K4F2) [NBP3-16424]

Immunocytochemistry/ Immunofluorescence: ACTR3 Antibody (8K4F2) [NBP3-16424]

Immunocytochemistry/Immunofluorescence: ACTR3 Antibody (8K4F2) [NBP3-16424] - Immunofluorescence analysis of U-2 OS cells using ACTR3 Rabbit mAb (NBP3-16424) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: ACTR3 Antibody (8K4F2) [NBP3-16424]

Immunohistochemistry-Paraffin: ACTR3 Antibody (8K4F2) [NBP3-16424]

Immunohistochemistry-Paraffin: ACTR3 Antibody (8K4F2) [NBP3-16424] - Immunohistochemistry of paraffin-embedded mouse spleen using ACTR3 Rabbit mAb (NBP3-16424) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for ACTR3 Antibody (8K4F2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ACTR3

The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion.

Long Name

Actin-related protein 3

Alternate Names

ARP3

Gene Symbol

ACTR3

Additional ACTR3 Products

Product Documents for ACTR3 Antibody (8K4F2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ACTR3 Antibody (8K4F2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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