ACTR3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23369
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Chicken, Xenopus
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for ACTR3 Antibody
Immunogen
ACTR3 (C-terminal region) synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human ACTR3. This peptide sequence is highly conserved in rat, mouse, chicken, fish, and yeast ACTR3 proteins. [UniProt# P61158]
Specificity
The antibody detects a 47 kDa doublet corresponding to the molecular mass of ACTR3 on SDS-PAGE immunoblots of human A431, Jurkat, and HeLa cells, and in rat PC12 cells.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for ACTR3 Antibody
Immunocytochemistry/Immunofluorescence: ACTR3 Antibody [NBP3-23369] -
Immunocytochemistry/Immunofluorescence: ACTR3 Antibody [NBP3-23369] - Immunocytochemical labeling of actin-related protein 2/3 complex subunit 1B in aldehyde-fixed and NP-40-permeabilized rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-actin-related protein 2/3 complex subunit 1B (C-terminal region) (NBP3-23369) in the absence (left) or presence (right) of blocking peptide. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.
Western Blot: ACTR3 Antibody [NBP3-23369] -
Western Blot: ACTR3 Antibody [NBP3-23369] - Western blot of human A431 (lane 1), Jurkat (lane 2), and HeLa (lane 3) cells. The blots were probed with rabbit polyclonal anti-actin-related protein 2/3 complex subunit 1B (C-terminal region) antibody at 1:1000 (lanes 1-3).Applications for ACTR3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:250
Immunohistochemistry
1:100
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: ACTR3
Long Name
Actin-related protein 3
Alternate Names
ARP3
Gene Symbol
ACTR3
Additional ACTR3 Products
Product Documents for ACTR3 Antibody
Product Specific Notices for ACTR3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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