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ACTR3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23369

Novus Biologicals, part of Bio-Techne
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NBP3-23369

Key Product Details

Species Reactivity

Human, Mouse, Rat, Chicken, Xenopus

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

ACTR3 (C-terminal region) synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human ACTR3. This peptide sequence is highly conserved in rat, mouse, chicken, fish, and yeast ACTR3 proteins. [UniProt# P61158]

Specificity

The antibody detects a 47 kDa doublet corresponding to the molecular mass of ACTR3 on SDS-PAGE immunoblots of human A431, Jurkat, and HeLa cells, and in rat PC12 cells.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for ACTR3 Antibody

ACTR3 Antibody

Immunocytochemistry/Immunofluorescence: ACTR3 Antibody [NBP3-23369] -

Immunocytochemistry/Immunofluorescence: ACTR3 Antibody [NBP3-23369] - Immunocytochemical labeling of actin-related protein 2/3 complex subunit 1B in aldehyde-fixed and NP-40-permeabilized rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-actin-related protein 2/3 complex subunit 1B (C-terminal region) (NBP3-23369) in the absence (left) or presence (right) of blocking peptide. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.
ACTR3 Antibody

Western Blot: ACTR3 Antibody [NBP3-23369] -

Western Blot: ACTR3 Antibody [NBP3-23369] - Western blot of human A431 (lane 1), Jurkat (lane 2), and HeLa (lane 3) cells. The blots were probed with rabbit polyclonal anti-actin-related protein 2/3 complex subunit 1B (C-terminal region) antibody at 1:1000 (lanes 1-3).

Applications for ACTR3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:250

Immunohistochemistry

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: ACTR3

The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion.

Long Name

Actin-related protein 3

Alternate Names

ARP3

Gene Symbol

ACTR3

Additional ACTR3 Products

Product Documents for ACTR3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ACTR3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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