ADAM10 Antibody (CL13808)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24577

Novus Biologicals, part of Bio-Techne
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NBP3-24577-100ul
NBP3-24577-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL13808

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ADAM10 Antibodies »

Product Specifications

Immunogen

This antibody was designed to target the recombinant protein ADAM10, with the precise immunogen sequence being withheld for proprietary reasons.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ADAM10 Antibody (CL13808)

Immunohistochemistry-Paraffin: ADAM10 Antibody (CL13808) [NBP3-24577]

Staining of human glioblastoma multiforme shows moderate to strong cytoplasmic positivity in tumor cells.

Immunohistochemistry-Paraffin: ADAM10 Antibody (CL13808) [NBP3-24577]

Analysis in human prostate and skeletal muscle tissues using NBP3-24577 antibody. Corresponding ADAM10 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: ADAM10 Antibody (CL13808) [NBP3-24577]

Staining of human kidney shows weak to moderate membranous positivity in cells in tubules.
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Applications for ADAM10 Antibody (CL13808)

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ADAM10

Proinflammatory cytokine tumor necrosis factor-alpha (TNF-a) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-a, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-a, notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively, (1,2,4). ADAM10 mRNA is expressed in a variety of human and bovine tissues (1,4).

Long Name

A Disintegrin and Metalloprotease-like Domain 10

Alternate Names

CD156c, Kuzbanian, MADM

Gene Symbol

ADAM10

Additional ADAM10 Products

Product Documents for ADAM10 Antibody (CL13808)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ADAM10 Antibody (CL13808)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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