ADAM10 Antibody (MM0077-6D31) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12014

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2 Clone # MM0077-6D31

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all ADAM10 Antibodies »

Product Specifications

Immunogen

Human recombinant ADAM10 (extracellular domain)

Specificity

In Western blots, this antibody shows weak cross-reactivity with recombinant mouse ADAM10 and human ADAM8, 9, 15, and 17.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2

Scientific Data Images for ADAM10 Antibody (MM0077-6D31) - Azide and BSA Free

Immunohistochemistry-Paraffin: ADAM10 Antibody (MM0077-6D31) - Azide and BSA Free [NBP2-12014]

Immunohistochemistry-Paraffin: ADAM10 Antibody (MM0077-6D31) - Azide and BSA Free [NBP2-12014]

Immunohistochemistry-Paraffin: ADAM10 Antibody (MM0077-6D31) [NBP2-12014] - 10% Buffer formalin fixed and parffine embedded human placetal tissue section (4um) is subjected IHC staining using NBP2-12014. Tissue section was pretreated in citric buffer (pH 6.0) with microwave for antige retrieval before IHC is applied.

Applications for ADAM10 Antibody (MM0077-6D31) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ADAM10

Proinflammatory cytokine tumor necrosis factor-alpha (TNF-a) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-a, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-a, notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively, (1,2,4). ADAM10 mRNA is expressed in a variety of human and bovine tissues (1,4).

Long Name

A Disintegrin and Metalloprotease-like Domain 10

Alternate Names

CD156c, Kuzbanian, MADM

Entrez Gene IDs

102 (Human)

Gene Symbol

ADAM10

UniProt

O14672

Additional ADAM10 Products

Product Documents for ADAM10 Antibody (MM0077-6D31) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ADAM10 Antibody (MM0077-6D31) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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