ADAM12 Antibody (1G3)

Novus Biologicals, part of Bio-Techne | Catalog # H00008038-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # 1G3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ADAM12 Antibodies »

Product Specifications

Immunogen

ADAM12 (NP_003465, 208 a.a. ~ 304 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ETLKATKYVELVIVADNREFQRQGKDLEKVKQRLIEIANHVDKFYRPLNIRIVLVGVEVWNDMDKCSVSQDPFTSLHEFLDWRKMKLLPRKSHDNAQ

Localization

Type I membrane protein (isoform 12L). A secreted form (isoform 12S) is produced by alternative splicing.

Specificity

ADAM12 - ADAM metallopeptidase domain 12 (meltrin alpha)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ADAM12 Antibody (1G3)

Western Blot: ADAM12 Antibody (1G3) [H00008038-M01]

Western Blot: ADAM12 Antibody (1G3) [H00008038-M01]

Western Blot: ADAM12 Antibody (1G3) [H00008038-M01] - Analysis of ADAM12 expression in transfected 293T cell line by ADAM12 monoclonal antibody (M01), clone 1G3.Lane 1: ADAM12 transfected lysate (Predicted MW: 80.4 KDa).Lane 2: Non-transfected lysate.
ELISA: ADAM12 Antibody (1G3) [H00008038-M01]

ELISA: ADAM12 Antibody (1G3) [H00008038-M01]

ELISA: ADAM12 Antibody (1G3) [H00008038-M01] - Detection limit for recombinant GST tagged ADAM12 is approximately 0.3ng/ml as a capture antibody.

Applications for ADAM12 Antibody (1G3)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ADAM12

This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis.

Long Name

A Disintegrin and Metalloprotease-like Domain 12

Alternate Names

Meltrin alpha

Entrez Gene IDs

8038 (Human)

Gene Symbol

ADAM12

OMIM

602714 (Human)

UniProt

NP_003465 (Human)

Additional ADAM12 Products

Product Documents for ADAM12 Antibody (1G3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ADAM12 Antibody (1G3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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