ADAM15 Antibody (007)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89546

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA Detection

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 007

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ADAM15 Antibodies »

Product Summary for ADAM15 Antibody (007)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human ADAM15 (Accession#: NP_997074.1; Met 1-Thr 696).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ADAM15 Antibody (007)

Immunocytochemistry/ Immunofluorescence: ADAM15 Antibody (007) [NBP2-89546]

Immunocytochemistry/ Immunofluorescence: ADAM15 Antibody (007) [NBP2-89546]

Immunocytochemistry/Immunofluorescence: ADAM15 Antibody (007) [NBP2-89546] - Staining of Human ADAM15 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ADAM15 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.
Immunohistochemistry-Paraffin: ADAM15 Antibody (007) [NBP2-89546]

Immunohistochemistry-Paraffin: ADAM15 Antibody (007) [NBP2-89546]

Immunohistochemistry-Paraffin: ADAM15 Antibody (007) [NBP2-89546] - Staining of human heart showing blood vessel staining (red arrow) (1:200).
Flow Cytometry: ADAM15 Antibody (007) [NBP2-89546]

Flow Cytometry: ADAM15 Antibody (007) [NBP2-89546]

Flow Cytometry: ADAM15 Antibody (007) [NBP2-89546] - Profile of anti-ADAM15 reactivity on MCF-7 cells.
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Applications for ADAM15 Antibody (007)

Application
Recommended Usage

ELISA

1:5000-1:10000

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunohistochemistry

1:1000-1:4000

Immunohistochemistry-Paraffin

1:1000-1:4000

Sandwich ELISA Detection

1:1000-1:10000
Application Notes
This antibody will detect Human ADAM15 in ELISA pair set (Catalog: # NBP2-79482). In a sandwich ELISA, it can be used as a detection antibody when paired with (Catalog: # NBP2-89548).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ADAM15

ADAM15 is encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.

Long Name

A Disintegrin and Metalloprotease-like Domain 15

Alternate Names

MDC15, Metargidin

Gene Symbol

ADAM15

Additional ADAM15 Products

Product Documents for ADAM15 Antibody (007)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ADAM15 Antibody (007)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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