ADAM15 Antibody (4T4X10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16642

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4T4X10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ADAM15 Antibodies »

Product Summary for ADAM15 Antibody (4T4X10)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ADAM15 (Q13444). RHIRRRRDVVTETKTVELVIVADHSEAQKYRDFQHLLNRTLEVALLLDTFFRPLNVRVALVGLEAWTQRDLVEISPNPAVTLENFLHWRRAHLLPRLPHDS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ADAM15 Antibody (4T4X10)

Western Blot: ADAM15 Antibody (4T4X10) [NBP3-16642]

Western Blot: ADAM15 Antibody (4T4X10) [NBP3-16642]

Western Blot: ADAM15 Antibody (4T4X10) [NBP3-16642] - Western blot analysis of extracts of HCT116 cells, using ADAM15 Rabbit mAb (NBP3-16642) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry: ADAM15 Antibody (4T4X10) [NBP3-16642]

Immunohistochemistry: ADAM15 Antibody (4T4X10) [NBP3-16642]

Immunohistochemistry: ADAM15 Antibody (4T4X10) [NBP3-16642] - Immunofluorescence analysis of mouse large intestine using ADAM15 Rabbit mAb (NBP3-16642) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: ADAM15 Antibody (4T4X10) [NBP3-16642]

Immunohistochemistry-Paraffin: ADAM15 Antibody (4T4X10) [NBP3-16642]

Immunohistochemistry-Paraffin: ADAM15 Antibody (4T4X10) [NBP3-16642] - Immunohistochemistry of paraffin-embedded rat lung using ADAM15 Rabbit mAb (NBP3-16642) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for ADAM15 Antibody (4T4X10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ADAM15

ADAM15 is encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.

Long Name

A Disintegrin and Metalloprotease-like Domain 15

Alternate Names

MDC15, Metargidin

Gene Symbol

ADAM15

Additional ADAM15 Products

Product Documents for ADAM15 Antibody (4T4X10)

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Certificate of Analysis

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Product Specific Notices for ADAM15 Antibody (4T4X10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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