ADAMTS5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15286

Novus Biologicals, part of Bio-Techne
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NBP2-15286
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Predicted:

Bovine (98%), Mouse (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ADAMTS5 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ADAMTS5. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (84%). .

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

102 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ADAMTS5 Antibody

Western Blot: ADAMTS5 Antibody [NBP2-15286]

Western Blot: ADAMTS5 Antibody [NBP2-15286]

Western Blot: ADAMTS5 Antibody [NBP2-15286] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAMTS5 antibody [N3C2], Internal diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: ADAMTS5 Antibody [NBP2-15286]

Immunohistochemistry-Paraffin: ADAMTS5 Antibody [NBP2-15286]

Immunohistochemistry-Paraffin: ADAMTS5 Antibody [NBP2-15286] - Human hepatoma. ADAMTS5 antibody [N3C2], Internal dilution: 1:250. Scale bar = 100 um. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: ADAMTS5 Antibody [NBP2-15286]

Western Blot: ADAMTS5 Antibody [NBP2-15286]

Western Blot: ADAMTS5 Antibody [NBP2-15286] - Sample (30 ug of whole cell lysate) A: HeLa B: HepG2 7. 5% SDS PAGE, antibody diluted at 1:3000.
View 1 more images

Applications for ADAMTS5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ADAMTS5

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene contains two C-terminal TS motifs and functions as aggrecanase to cleave aggrecan, a major proteoglycan of cartilage. [provided by RefSeq]

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 5

Alternate Names

ADAMTS11, ADMP-2, Aggrecanase 2

Gene Symbol

ADAMTS5

UniProt

Q9UNA0

Additional ADAMTS5 Products

Product Documents for ADAMTS5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ADAMTS5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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