ADAR Antibody (7H1U2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15383
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7H1U2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for ADAR Antibody (7H1U2)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 200-300 of human ADAR (P55265). STQAWNQHSGVVRPDGHSQGAPNSDPSLEPEDRNSTSVSEDLLEPFIAVSAQAWNQHSGVVRPDSHSQGSPNSDPGLEPEDSNSTSALEDPLEFLDMAEIK
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ADAR Antibody (7H1U2)
Western Blot: ADAR Antibody (7H1U2) [NBP3-15383]
Western Blot: ADAR Antibody (7H1U2) [NBP3-15383] - Analysis of extracts of various cell lines, using ADAR Rabbit mAb (NBP3-15383) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Immunohistochemistry-Paraffin: ADAR Antibody (7H1U2) [NBP3-15383]
Immunohistochemistry-Paraffin: ADAR Antibody (7H1U2) [NBP3-15383] - Mouse kidney using ADAR Rabbit mAb (NBP3-15383) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Immunohistochemistry-Paraffin: ADAR Antibody (7H1U2) [NBP3-15383]
Immunohistochemistry-Paraffin: ADAR Antibody (7H1U2) [NBP3-15383] - Rat ovary using ADAR Rabbit mAb (NBP3-15383) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for ADAR Antibody (7H1U2)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ADAR
Long Name
Double-stranded RNA-specific adenosine deaminase
Alternate Names
ADAR1, DRADA, DSRAD, EC 3.5.4.37, G1P1, IFI-4, IFI4, K88DSRBP, p136
Gene Symbol
ADAR
Additional ADAR Products
Product Documents for ADAR Antibody (7H1U2)
Product Specific Notices for ADAR Antibody (7H1U2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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