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ADCK4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85510

Novus Biologicals, part of Bio-Techne
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NBP1-85510
NBP1-85510-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ACAQNFRQLLANDPFFRVPAVVKELCTTRVLGMELAGGVPLDQCQGLSQDLRNQICFQLLTLCLR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ADCK4 Antibody

Western Blot: ADCK4 Antibody [NBP1-85510]

Western Blot: ADCK4 Antibody [NBP1-85510]

Western Blot: ADCK4 Antibody [NBP1-85510] - Analysis in control (vector only transfected HEK293T lysate) and ADCK4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: ADCK4 Antibody [NBP1-85510]

Immunocytochemistry/ Immunofluorescence: ADCK4 Antibody [NBP1-85510]

Immunocytochemistry/Immunofluorescence: ADCK4 Antibody [NBP1-85510] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria. antibody staining is shown in green.
Immunohistochemistry-Paraffin: ADCK4 Antibody [NBP1-85510]

Immunohistochemistry-Paraffin: ADCK4 Antibody [NBP1-85510]

Immunohistochemistry-Paraffin: ADCK4 Antibody [NBP1-85510] - Staining of human kidney shows moderate cytoplasmic positivity and distinct staining of luminal membranes in tubular cells.

Applications for ADCK4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ADCK4

The function of this protein is not yet clear. It is not known if it has protein kinase activity and whattype of substrate it would phosphorylate (Ser, Thr or Tyr)

Alternate Names

aarF domain containing kinase 4, COQ8, EC 2.7.11, EC 2.7.11.-, EC 2.7.11.1, EC 2.7.11.25, FLJ12229

Gene Symbol

COQ8B

Additional ADCK4 Products

Product Documents for ADCK4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ADCK4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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