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ADCK5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83500

Novus Biologicals, part of Bio-Techne
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NBP1-83500
NBP1-83500-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VLLDHGLYQFLEEKDRAALCQLWRAIILRDDAAMRAHAAALGVQDYLLFAEMLMQRPVRLGQLWGSHLLSREEAAYMVDMARERFEAVMAVLRELP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ADCK5 Antibody

Western Blot: ADCK5 Antibody [NBP1-83500]

Western Blot: ADCK5 Antibody [NBP1-83500]

Western Blot: ADCK5 Antibody [NBP1-83500] - Analysis in control (vector only transfected HEK293T lysate) and ADCK5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: ADCK5 Antibody [NBP1-83500]

Immunocytochemistry/ Immunofluorescence: ADCK5 Antibody [NBP1-83500]

Immunocytochemistry/Immunofluorescence: ADCK5 Antibody [NBP1-83500] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: ADCK5 Antibody [NBP1-83500]

Immunohistochemistry-Paraffin: ADCK5 Antibody [NBP1-83500]

Immunohistochemistry-Paraffin: ADCK5 Antibody [NBP1-83500] - Staining of human pancreas shows strong cytoplasmic positivity in islet cells.

Applications for ADCK5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ADCK5

The function of this protein is not yet clear. It is not known if it has protein kinase activity and whattype of substrate it would phosphorylate (Ser, Thr or Tyr)

Alternate Names

aarF domain containing kinase 5, EC 2.7.11.-, FLJ35454, MGC126708

Entrez Gene IDs

203054 (Human)

Gene Symbol

ADCK5

UniProt

Additional ADCK5 Products

Product Documents for ADCK5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ADCK5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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