Adenosine A1R Antibody (10D12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15218

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15218
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10D12

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

A synthesized peptide derived from human Adenosine A1R. UniProt ID (P30542).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Adenosine A1R Antibody (10D12)

Western Blot: Adenosine A1R Antibody (10D12) [NBP3-15218]

Western Blot: Adenosine A1R Antibody (10D12) [NBP3-15218]

Western Blot: Adenosine A1R Antibody (10D12) [NBP3-15218] - Positive WB detected in: A549 whole cell lysate, 293 whole cell lysate, U87 whole cell lysate. All lanes: Adenosine A1R antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 37, 14 KDa. Observed band size: 37 KDa.
Immunohistochemistry: Adenosine A1R Antibody (10D12) [NBP3-15218]

Immunohistochemistry: Adenosine A1R Antibody (10D12) [NBP3-15218]

Immunohistochemistry: Adenosine A1R Antibody (10D12) [NBP3-15218] - IHC image of Adenosine A1R antibody diluted at 1:100 and staining in paraffin-embedded human brain tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Applications for Adenosine A1R Antibody (10D12)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, and 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Adenosine A1R

Adenosine receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily. Structural, biochemical and pharmacological analyses of the AR genes and protein has led to the discovery of four distinct AR subtypes (A1, A2a, A2b, A3). Activation of ARs mediates several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission.The A1AR is a glycoprotein that can activate Gi and Go proteins in vitro. In intact cells, agonist occupation of the A1AR has been shown to cause pertussis toxin-sensitive inhibition of adenylyl cyclase activity and, in some systems, a stimulation of phospholipase C resulting in mobilization of intracellular calcium stores. Activation of potassium channels by A1AR has been intensively studied in relation to its dramatic effects on the cardiovascular system. A1AR protein is highly expressed in brain (especially cerebellum, hippocampus, thalamus, and cortex) and spinal cord and in part, modulates neurotransmitter release. In white adipocytes A1AR inhibits lipolysis and stimulates glucose uptake. Other tissues also express A1AR including kidney and testis.

Long Name

Adenosine A1 Receptor

Alternate Names

Adenosine A1 R, ADORA1, RDC7

Gene Symbol

ADORA1

Additional Adenosine A1R Products

Product Documents for Adenosine A1R Antibody (10D12)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Adenosine A1R Antibody (10D12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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