Adenosine A1R Antibody (JE31-35)

Immunohistochemistry: Adenosine A1R Antibody (JE31-35) [NBP3-32002]

Immunohistochemistry: Adenosine A1R Antibody (JE31-35) [NBP3-32002] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Adenosine A1R antibody (NBP3-32002) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32002) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: Adenosine A1R Antibody (JE31-35) [NBP3-32002]

Western Blot: Adenosine A1R Antibody (JE31-35) [NBP3-32002] - Western blot analysis of Adenosine A1R on different lysates with Rabbit anti-Adenosine A1R antibody (NBP3-32002) at 1/1,000 dilution.

Lane 1: A549 cell lysate (15 ug/Lane)
Lane 2: HEK-293 cell lysate (15 ug/Lane)
Lane 3: U-87 MG cell lysate (15 ug/Lane)
Lane 4: SH-SY5Y cell lysate (15 ug/Lane)
Lane 5: HeLa cell lysate (15 ug/Lane)
Lane 6: Raji cell lysate (15 ug/Lane)
Lane 7: NIH/3T3 cell lysate (15 ug/Lane)
Lane 8: Human brain tissue lysate (30 ug/Lane)
Lane 9: Mouse brain tissue lysate (30 ug/Lane)
Lane 10: Rat brain tissue lysate (30 ug/Lane)
Lane 11: Human cerebellum tissue lysate (30 ug/Lane)
Lane 12: Rat cerebellum tissue lysate (30 ug/Lane)

Predicted band size: 36.5 kDa
Observed band size: 36.5/40 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32002) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.