Adenosine A1R Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB300-549

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Adenosine A1R Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues C(309) Q P K P P I D E D L P E E K A E D(326) of rat Adenosine Receptor A1.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 18948751). Rat from customer review.

Localization

Cell Membrane

Specificity

Detects Adenosine Receptor A1. This does not detect other AR subtypes.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Adenosine A1R Antibody - BSA Free

Western Blot: Adenosine A1R AntibodyBSA Free [NB300-549]

Western Blot: Adenosine A1R AntibodyBSA Free [NB300-549]

Western Blot: Adenosine A1R Antibody [NB300-549] - Analysis was performed on whole cell extracts (30 ug lysate) of PC 12 (Lane 1), SH-SY5Y (lane 2), U-87MG (lane 3), A549 (Lane 4) and K-562 (lane 5).
Immunocytochemistry/ Immunofluorescence: Adenosine A1R Antibody - BSA Free [NB300-549]

Immunocytochemistry/ Immunofluorescence: Adenosine A1R Antibody - BSA Free [NB300-549]

Immunocytochemistry/Immunofluorescence: Adenosine A1R Antibody [NB300-549] - Analysis of Adenosine Receptor A1 was done on 70% confluent log phase HeLa cells.
Immunohistochemistry-Paraffin: Adenosine A1R Antibody - BSA Free [NB300-549]

Immunohistochemistry-Paraffin: Adenosine A1R Antibody - BSA Free [NB300-549]

Immunohistochemistry-Paraffin: Adenosine A1R Antibody [NB300-549] - Analysis showing positive staining in the cytoplasm of Rat brain tissue (right) compared with a negative control in the absence of primary antibody (left).
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Applications for Adenosine A1R Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

3 - 5 ug / 10^6 cells

Immunocytochemistry/ Immunofluorescence

2 ug/ml

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500
Application Notes
Adenosine A1R antibody validated for ICC/IF from a verified customer review. Use in IHC- Frozen reported in scientific literature (PMID: 24016034).

Reviewed Applications

Read 1 review rated 4 using NB300-549 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Adenosine A1R

Adenosine receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily. Structural, biochemical and pharmacological analyses of the AR genes and protein has led to the discovery of four distinct AR subtypes (A1, A2a, A2b, A3). Activation of ARs mediates several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission.The A1AR is a glycoprotein that can activate Gi and Go proteins in vitro. In intact cells, agonist occupation of the A1AR has been shown to cause pertussis toxin-sensitive inhibition of adenylyl cyclase activity and, in some systems, a stimulation of phospholipase C resulting in mobilization of intracellular calcium stores. Activation of potassium channels by A1AR has been intensively studied in relation to its dramatic effects on the cardiovascular system. A1AR protein is highly expressed in brain (especially cerebellum, hippocampus, thalamus, and cortex) and spinal cord and in part, modulates neurotransmitter release. In white adipocytes A1AR inhibits lipolysis and stimulates glucose uptake. Other tissues also express A1AR including kidney and testis.

Long Name

Adenosine A1 Receptor

Alternate Names

Adenosine A1 R, ADORA1, RDC7

Entrez Gene IDs

29290 (Rat)

Gene Symbol

ADORA1

UniProt

P25099 (Rat)

Additional Adenosine A1R Products

Product Documents for Adenosine A1R Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Adenosine A1R Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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