Key Product Details

Species Reactivity

Validated:

Adenovirus, Virus

Cited:

Human, Mouse, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Virus

Applications

Validated:

Block/Neutralize, ELISA, Immunocytochemistry/ Immunofluorescence, Immunodiffusion, Immunohistochemistry, Lateral Flow Assay, Sandwich ELISA

Cited:

Block/Neutralize, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 8C4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Adenovirus Antibodies »

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Human adenovirus type 1 and canine adenovirus type 1.

Specificity

Hexon antigen of human adenovirus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Adenovirus Antibody (8C4)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Sandwich ELISA

1:100-1:2000
Application Notes
Recommended pair for sandwich ELISA (capture-detection): NB600-413 - NB200-425. This antibody has been reported in the literature to work in Immunohistochemistry-Frozen sections (PMID: 19622582). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 26861248). Use in Block/Neutralize reported in scientific literature (PMID:31848338). This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Adenovirus

Adenoviruses are DNA viruses generally widespread in nature that are frequently the cause of acute upper respiratory tract infections (i.e. common colds). Forty-seven known serotypes have been isolated since they were first discovered in 1953 with 3 types known to cause gastroenteritis. Several types have oncogenic potential though most cause self-limiting febrile illnesses characterized by inflammation of conjunctivae and the respiratory tract. The virus can be isolated from the majority of tonsils/adenoids surgically removed, indicating latent infections. It is not known how long the virus can persist in the body, or whether it is capable of reactivation after long periods. In patients experiencing immunosuppression (e.g. AIDS) it can be reactivated causing disease.

Alternate Names

Adenovirus

Additional Adenovirus Products

Product Documents for Adenovirus Antibody (8C4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Adenovirus Antibody (8C4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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