Adenylate Cyclase 3 Antibody (1A12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05526

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1A12

Concentration

1 mg/ml

View all Adenylate Cyclase 3 Antibodies »

Product Specifications

Immunogen

C-terminal peptide of rat Adenylate Cyclase 3, PAAFPNGSSVTLPHQVVDNP with a Cys added to the N-terminus to allow coupling to KLH.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Adenylate Cyclase 3 Antibody (1A12)

Western Blot: Adenylate Cyclase 3 Antibody [NBP3-05526]

Western Blot: Adenylate Cyclase 3 Antibody [NBP3-05526]

Western Blot: Adenylate Cyclase 3 Antibody [NBP3-05526] - Western blot analysis of HEK293 cell lysates using Adenylate Cyclase 3 Antibody, dilution 1:1,000, in green: [1] protein standard, [2] non-transfected HEK293 cells, and [3] HEK293 cells transfected with a vector encoding Myc-DDK tagged fill length human adenylate cyclase 3. The strong band at about 130kDa in transfected cells demonstrates overexpression of the human adenylate cyclase 3 protein, and bands over 250kDa presumably correspond to either heavily glycosolated form of adenylate cyclase 3 or multimeric forms of the protein. The same blot was simultaneously probed with rabbit polyclonal antibody to GAPDH dilution 1:20,000 in red, which reveals the single band at ~37kDa seen in both transfected and non-transfected cells.
Immunocytochemistry/ Immunofluorescence: Adenylate Cyclase 3 Antibody [NBP3-05526]

Immunocytochemistry/ Immunofluorescence: Adenylate Cyclase 3 Antibody [NBP3-05526]

Immunocytochemistry/Immunofluorescence: Adenylate Cyclase 3 Antibody [NBP3-05526] - Immunofluoresence of caudate/putamen region of rat brain stained with Adenylate Cyclase 3 Antibody in red, and chicken antibody to tyrosine hydroxylase, in green. The Blue is Hoechst stain revealing nuclei. The Adenylate Cyclase 3 Antibody reveals neuronal cilia while the tyrosine hydroxylase antibody reveals the axons of chatecholaminergic neurons.

Applications for Adenylate Cyclase 3 Antibody (1A12)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: Adenylate Cyclase 3

ADCY3, also known as Adenylate cyclase type 3, is a 129 kDa 1,144 amino acid protein, and is involved in the formation of the secondary messenger protein, cAMP. This protein has also been shown to have interactions with STOML3, CAMK2G, PDE1A, PDE1C, and PDE2A in the Signaling by FGFR, PKA activation in glucagon signaling, DAG and IP3 signaling, Regulation of Water Balance by Renal Aquaporins, and Opioid Signalling pathway. Disease research is currently being studied with relation to ADCY3 and cholera, alcoholism, tuberculosis, and breast cancer.

Long Name

Adenylate cyclase type 3

Alternate Names

AC-III, AC3, ADCY3, Adenylyl cyclase 3, KIAA0511

Gene Symbol

ADCY3

Additional Adenylate Cyclase 3 Products

Product Documents for Adenylate Cyclase 3 Antibody (1A12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Adenylate Cyclase 3 Antibody (1A12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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