Adenylate Cyclase 3 Antibody [DyLight 405]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92683V

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Polyclonal Rabbit

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Adenylate Cyclase 3 Antibodies »

Product Specifications

Immunogen

C-terminal peptide of rat ACIII, PAAFPNGSSVTLPHQVVDNP with a Cys added to the N-terminus to allow coupling to KLH.

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34572121). .

Localization

Membrane; Multi-pass membrane protein.

Clonality

Polyclonal

Host

Rabbit

Description

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Applications for Adenylate Cyclase 3 Antibody [DyLight 405]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This Adenylate Cyclase 3 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where bands can be seen at 160-200 kDa (depending on the tissue). In WB, bands can be seen at about 200kDa in olfactory epithelium which is rich in cilia. Fewer cilia are found in frontal cortex, and the protein is less heavily glycosylated, and a less prominent band is seen at about 160kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Adenylate Cyclase 3

The biosynthesis of cAMP/cyclic 3'5'-AMP from ATP is catalyzed by adenylyl cyclases (ADCYs or ACs) which are a groups of enzymes having about ten closely related isoforms including ADCYs 1-9 and ADCYAP1 (ADCY activating polypeptide 1). Adenylate Cyclase 3 (ADCY3 or AC3) is third member of ADCYs family which is a membrane-associated enzyme and catalyzes the formation of secondary messenger cAMP. ADCY3 enzyme is localized in the membranes surrounding cilia in neurons, and is considered an excellent marker of neuronal cilia in brain tissues or in cell culture. Human ADCY3 is a large complex molecule of 1145 amino acids with an expected molecular weight of 129kD and due to extensive glycosylation, a diffused band of about 160kD in cortex and about 200kD in olfactory epithelium is generally observed in Western blots. ADCY3 appears to be widely expressed in various human tissues wherein it implicates in a number of physiological (including odorant detection via controlling intracellular cAMP levels) as well as pathophysiological metabolic processes. ADCY3 knock out mice exhibits obesity upon ageing primarily because of low locomoter activity, hyperphagia and leptin insensitivity. Moreover, ADCY3 genetic polymorphisms have been linked to decreased risk of body mass index/BMI among the subjects with obesity and type 2 diabetes/NIDDM suggesting that ADCY3 is a novel candidate in the regulation of body weight.

Long Name

Adenylate cyclase type 3

Alternate Names

AC-III, AC3, ADCY3, Adenylyl cyclase 3, KIAA0511

Gene Symbol

ADCY3

Additional Adenylate Cyclase 3 Products

Product Documents for Adenylate Cyclase 3 Antibody [DyLight 405]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Adenylate Cyclase 3 Antibody [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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