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Adenylate Kinase 1 Antibody (7E9) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59485

Novus Biologicals, part of Bio-Techne
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NBP2-59485-100ul
NBP2-59485-50ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 7E9

Format

BSA Free

Concentration

1 mg/ml

Product Summary for Adenylate Kinase 1 Antibody (7E9) - BSA Free

Immunogen

Recombinant human Adenylate Kinase 1 (1-194aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Adenylate Kinase 1 Antibody (7E9) - BSA Free

Western Blot: Adenylate Kinase 1 Antibody (7E9)BSA Free [NBP2-59485]

Western Blot: Adenylate Kinase 1 Antibody (7E9)BSA Free [NBP2-59485]

Western Blot: Adenylate Kinase 1 Antibody (7E9) [NBP2-59485] - Lane 1: HeLa cell lysates, Lane 2: U87-MG cell lysates, Lane 3: K562 cell lysates, Lane 4: 293T cell lysates, Lane 5: HepG2 cell lysates, Lane 6: A549 cell lysates, Lane 7: MCF7cell lysates, Lane 8: SK-OV-3 cell lysates, Lane 9: PC3 cell lysates
Immunocytochemistry/ Immunofluorescence: Adenylate Kinase 1 Antibody (7E9) - BSA Free [NBP2-59485]

Immunocytochemistry/ Immunofluorescence: Adenylate Kinase 1 Antibody (7E9) - BSA Free [NBP2-59485]

Immunocytochemistry/Immunofluorescence: Adenylate Kinase 1 Antibody (7E9) [NBP2-59485] - Analysis of AK1 in HeLa cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of AK1 in Hep3B cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of AK1 in A549 cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Applications for Adenylate Kinase 1 Antibody (7E9) - BSA Free

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Adenylate Kinase 1

Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.

Long Name

Adenylate kinase isoenzyme 1

Alternate Names

AK 1, AK1, EC 2.7.4.3, EC 2.7.4.6, Myokinase

Gene Symbol

AK1

Additional Adenylate Kinase 1 Products

Product Documents for Adenylate Kinase 1 Antibody (7E9) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Adenylate Kinase 1 Antibody (7E9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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