Adipogenesis Antibody Pack
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20190
Key Product Details
Species
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Product Summary for Adipogenesis Antibody Pack
This pack contains 1 vial each of: MAB6898-SP (0.025 mg), NB600-582SS (0.025 mg), MAB7094-SP (0.025 mg), AF3150-SP (0.025 mg), NB400-144SS (0.025 mg), NB110-40760SS (0.025 mL), NBP2-22106SS (0.025 ml), AF2989-SP (0.025 mg), HAF017 (1.0 ml), HAF008 (1.0 ml), and HAF007 (1.0 ml).
Product Specifications
Application Notes
See individual datasheets of components for their validated applications
Reactivity Notes
See individual datasheets of components for their validated species
Scientific Data Images for Adipogenesis Antibody Pack
Western Blot: Adipogenesis Antibody Pack [NBP3-20190]
Western Blot: Adipogenesis Antibody Pack [NBP3-20190] - Western blot shows lysates of human heart tissue, rat heart tissue, mouse heart tissue, human adipose tissue, rat adipose tissue, and mouse adipose tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for FABP4/A-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot: Adipogenesis Antibody Pack [NBP3-20190]
Western Blot: Adipogenesis Antibody Pack [NBP3-20190] - Western Blot: CD36 Antibody [NB400-144] - Total protein from Human Skin and Adipose tissue, Mouse Adipose and Rat Adipose tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-CD36 in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Flow Cytometry: Adipogenesis Antibody Pack [NBP3-20190]
Flow Cytometry: Adipogenesis Antibody Pack [NBP3-20190] - An intracellular stain was performed on U2OS cells with Perilipin Antibody NB110-40760AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.Kit Contents for Adipogenesis Antibody Pack
- AF2989-SP
- AF3150-SP
- HAF017: Goat IgG HRP-conjugated Antibody
- MAB6898-SP
- MAB7094-SP
- HAF007: Mouse IgG Horseradish Peroxidase-conjugated Antibody
- NB110-40760SS
- NB400-144SS
- NB600-582SS
- NBP2-22106SS
- HAF008: Rabbit IgG Horseradish Peroxidase-conjugated Antibody
Formulation, Preparation, and Storage
Concentration
Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Storage is content dependent.
Background: Adipogenesis
Additional Adipogenesis Products
Product Specific Notices for Adipogenesis Antibody Pack
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.
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