ADNP Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97747
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human ADNP fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for ADNP Antibody
Western Blot: ADNP Antibody [NBP2-97747] - Anti-ADNP rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane.
Western Blot: ADNP Antibody [NBP2-97747] - Anti-ADNP rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane.![Immunocytochemistry/ Immunofluorescence: ADNP Antibody [NBP2-97747]](https://resources.bio-techne.com/images/products/ADNP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97747-img0001.jpg "Immunocytochemistry/ Immunofluorescence: ADNP Antibody [NBP2-97747]")
Immunocytochemistry/ Immunofluorescence: ADNP Antibody [NBP2-97747]
Immunocytochemistry/Immunofluorescence: ADNP Antibody [NBP2-97747] - Immunofluorescence staining of ADNP in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ADNP polyclonal antibody (dilution ratio 1:300) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG Secondary antibody (red). Positive staining was localized to Nucleus.![Immunoprecipitation: ADNP Antibody [NBP2-97747]](https://resources.bio-techne.com/images/products/ADNP-Antibody-Immunoprecipitation-NBP2-97747-img0004.jpg "Immunoprecipitation: ADNP Antibody [NBP2-97747]")
Immunoprecipitation: ADNP Antibody [NBP2-97747]
Immunoprecipitation: ADNP Antibody [NBP2-97747] - ADNP was immunoprecipitated using: Lane A: 0.5 mg U-251MG Whole Cell Lysate. 4 ul anti-ADNP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-ADNP rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 122 kDa. Observed band size: 150 kDa.Applications for ADNP Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:200-1:1000
Immunoprecipitation
5-10 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ADNP
Long Name
Activity-dependent Neuroprotective Protein
Alternate Names
ADNP1
Gene Symbol
ADNP
Additional ADNP Products
Product Specific Notices for ADNP Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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