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ADP-Sugar Pyrophosphatase/NUDT5 Antibody (2A3)

Novus Biologicals, part of Bio-Techne | Catalog # H00011164-M04

Novus Biologicals, part of Bio-Techne
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H00011164-M04

Key Product Details

Species Reactivity

Human

Applications

ELISA, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2A3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

NUDT5 (NP_054861, 120 a.a. ~ 219 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KGDIAECSPAVCMDPGLSNCTIHIVTVTINGDDAENARPKPKPGDGEFVEVISLPKNDLLQRLDALVAEEHLTVDARVYSYALALKHANAKPFEVPFLKF

Reactivity Notes

Human. Other species not tested.

Specificity

NUDT5 (2A3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for ADP-Sugar Pyrophosphatase/NUDT5 Antibody (2A3)

Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody (2A3) [H00011164-M04]

Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody (2A3) [H00011164-M04]

Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody (2A3) [H00011164-M04] - Analysis of NUDT5 expression in transfected 293T cell line by NUDT5 monoclonal antibody (M04), clone 2A3. Lane 1: NUDT5 transfected lysatE (24.3 KDa). Lane 2: Non-transfected lysate.
Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody (2A3) [H00011164-M04]

Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody (2A3) [H00011164-M04]

Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody (2A3) [H00011164-M04] - Western blot analysis of NUDT5 over-expressed 293 cell line, cotransfected with NUDT5 Validated Chimera RNAi or non-transfected control. Blot probed with H00011164-M04. GAPDH (36.1 kDa) used as loading control.
Sandwich ELISA: ADP-Sugar Pyrophosphatase/NUDT5 Antibody (2A3) [H00011164-M04] - Detection limit for recombinant GST tagged NUDT5 is approximately 1ng/ml as a capture antibody.

Applications for ADP-Sugar Pyrophosphatase/NUDT5 Antibody (2A3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ADP-Sugar Pyrophosphatase/NUDT5

Nudix hydrolases, such as NUDT5, eliminate toxic nucleotide derivatives from the cell and regulate the levels of important signaling nucleotides and their metabolites (McLennan, 1999 [PubMed 10373642]).[supplied by OMIM]

Alternate Names

Nudix motif 5, YSA1, YSA1H

Entrez Gene IDs

11164 (Human)

Gene Symbol

NUDT5

OMIM

609230 (Human)

Additional ADP-Sugar Pyrophosphatase/NUDT5 Products

Product Documents for ADP-Sugar Pyrophosphatase/NUDT5 Antibody (2A3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ADP-Sugar Pyrophosphatase/NUDT5 Antibody (2A3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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