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ADPRHL1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-39073

Novus Biologicals, part of Bio-Techne
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NBP2-39073

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: ALFVSFAAQGKPLVQWGRDMLRAVPLAEEYCRKTIRHTAEYQEHWFYFEAKWQFYLEERKISKDSENKAIFPDNYDAEEREKTYRKWSSEGR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ADPRHL1 Antibody

Western Blot: ADPRHL1 Antibody [NBP2-39073]

Western Blot: ADPRHL1 Antibody [NBP2-39073]

Western Blot: ADPRHL1 Antibody [NBP2-39073] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human Skeletal muscle tissue
Immunocytochemistry/ Immunofluorescence: ADPRHL1 Antibody [NBP2-39073]

Immunocytochemistry/ Immunofluorescence: ADPRHL1 Antibody [NBP2-39073]

Immunocytochemistry/Immunofluorescence: ADPRHL1 Antibody [NBP2-39073] - Staining of human cell line MCF7 shows localization to nucleoplasm. Antibody stainign is shown in green.
Immunohistochemistry-Paraffin: ADPRHL1 Antibody [NBP2-39073]

Immunohistochemistry-Paraffin: ADPRHL1 Antibody [NBP2-39073]

Immunohistochemistry-Paraffin: ADPRHL1 Antibody [NBP2-39073] - Staining in human heart muscle and prostate tissues using anti-ADPRHL1 antibody. Corresponding ADPRHL1 RNA-seq data are presented for the same tissues.

Applications for ADPRHL1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ADPRHL1

ADP-ribosylation is a reversible posttranslational modification used to regulate protein function. ADP-ribosyltransferases (see ART1; MIM 601625) transfer ADP-ribose from NAD+ to the target protein, and ADP-ribosylhydrolases, such as ADPRHL1, reverse the reaction (Glowacki et al., 2002 [PubMed 12070318]).[supplied by OMIM]

Alternate Names

[Protein ADP-ribosylarginine] hydrolase-like protein 1, ADP-ribosyl-hydrolase, ADP-ribosylhydrolase like 1, ARH2ADP-ribosylhydrolase 2

Gene Symbol

ADPRHL1

UniProt

Additional ADPRHL1 Products

Product Documents for ADPRHL1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ADPRHL1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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