ADTB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99079

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ADTB1 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human ADTB1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ADTB1 Antibody

Western Blot: ADTB1 Antibody [NBP2-99079]

Western Blot: ADTB1 Antibody [NBP2-99079]

Western Blot: ADTB1 Antibody [NBP2-99079] - Anti-ADTB1 rabbit polyclonal antibody at 1:500 dilution. Lane A: NIH3T3 Whole Cell Lysate. Lysates/proteins at 10 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 105 kDa. Observed band size: 105 kDa
Immunoprecipitation: ADTB1 Antibody [NBP2-99079]

Immunoprecipitation: ADTB1 Antibody [NBP2-99079]

Immunoprecipitation: ADTB1 Antibody [NBP2-99079] - ADTB1 was immunoprecipitated using: Lane A: 0.5 mg NIH-3T3 Whole Cell Lysate. Lane B: 0.5 mg K562 Whole Cell Lysate. Lane C: 0.5 mg Hela Whole Cell Lysate. 1 ul anti-ADTB1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-ADTB1 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 105 kDa. Observed band size: 112 kDa

Applications for ADTB1 Antibody

Application
Recommended Usage

Immunoprecipitation

0.1-0.5 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ADTB1

Membrane vesicle formation is a process required for the endocytosis and biosynthesis of various secreted and membrane bound proteins. Clathrin is a protein which assembles into a polyhedral network on the cell membrane as the membrane invaginates, forming a coated pit which is essential to endocytosis. Clathrin is composed of three polypeptides, a 180 kDa heavy chain and two 32-38 kDa light chains which combine to create a distinct three-legged triskelion. It is this morphology which allows Clathrin to form its unique polyhedral network.

Alternate Names

Adapter-Related Protein Complex 1 Subunit Beta-1, Adaptor Protein Complex AP-1 Subunit Beta-1, Adaptor-Related Protein Complex 1, Beta 1 Subunit, AP-1 Complex Subunit Beta-1, AP105A, AP1B1, BAM22, Beta-1-adaptin, beta1-adaptin, Beta-Adaptin 1, beta-prime-adaptin, CLAPB2, Clathrin Assembly Protein Complex 1 Beta Large Chain, Golgi Adaptor HA1/AP1 Adaptin Beta Subunit, Plasma Membrane Adaptor HA2/AP2 Adaptor Beta Subunit

Gene Symbol

AP1B1

Additional ADTB1 Products

Product Documents for ADTB1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ADTB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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