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AE Binding Protein 1/ACLP Antibody (1D2)

Novus Biologicals, part of Bio-Techne | Catalog # H00000165-M01

Novus Biologicals, part of Bio-Techne
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H00000165-M01

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1D2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

AEBP1 (NP_001120, 912 a.a. ~ 1013 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VTDEQGIPIANATISVSGINHGVKTASGGDYWRILNPGEYRVTAHAEGYTPSAKTCNVDYDIGATQCNFILARSNWKRIREIMAMNGNRPIPHIDPSRPMTP

Specificity

AEBP1 - AE binding protein 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for AE Binding Protein 1/ACLP Antibody (1D2)

Immunohistochemistry-Paraffin: AE Binding Protein 1/ACLP Antibody (1D2) [H00000165-M01]

Immunohistochemistry-Paraffin: AE Binding Protein 1/ACLP Antibody (1D2) [H00000165-M01]

Immunohistochemistry-Paraffin: AE Binding Protein 1/ACLP Antibody (1D2) [H00000165-M01] - Analysis of monoclonal antibody to AEBP1 on formalin-fixed paraffin-embedded human adrenal gland. Antibody concentration 3 ug/ml.
ELISA: AE Binding Protein 1/ACLP Antibody (1D2) [H00000165-M01]

ELISA: AE Binding Protein 1/ACLP Antibody (1D2) [H00000165-M01]

ELISA: AE Binding Protein 1/ACLP Antibody (1D2) [H00000165-M01] - Detection limit for recombinant GST tagged AEBP1 is approximately 0.3ng/ml as a capture antibody.

Applications for AE Binding Protein 1/ACLP Antibody (1D2)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA. Use in Immunohistochemistry reported in scientific literature (PMID: 30097586).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ACLP

The adipocyte enhancer binding protein 1 is a transcriptional repressor with carboxypeptidase (CP) activity. This protein binds to a regulatory sequence, adipocyte enhancer 1 (AE-1), located in the proximal promoter region of the adipose P2 (aP2) gene, which encodes the adipocyte fatty-acid binding protein. It is characterized as a member of the regulatory B-like CP family. This protein seems to be activated by a novel mechanism, whereby the direct binding of DNA enhances its protease activity. Adipocyte-enhancer binding protein 1 may play a role in differentiated vascular smooth muscle cells.

Long Name

Aortic Carboxypeptidase-like Protein

Alternate Names

AEBP1

Entrez Gene IDs

165 (Human)

Gene Symbol

AEBP1

OMIM

602981 (Human)

UniProt

Additional ACLP Products

Product Documents for AE Binding Protein 1/ACLP Antibody (1D2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AE Binding Protein 1/ACLP Antibody (1D2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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