AF4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28705
![Immunoprecipitation: AF4 Antibody [NBP1-28705]](https://resources.bio-techne.com/images/products/AF4-Antibody-Immunoprecipitation-NBP1-28705-img0003.jpg "Immunoprecipitation: AF4 Antibody [NBP1-28705]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Validated:
Immunoprecipitation, Western Blot (Negative)
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 175 and 225 of human proto-oncogene AF4 using the numbering given in entry NP_005926.1 (GeneID 4299).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for AF4 Antibody
Immunoprecipitation: AF4 Antibody [NBP1-28705]
Immunoprecipitation: AF4 Antibody [NBP1-28705] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-AF4 antibody NBP1-28728 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. AF4 was also immunoprecipitated by rabbit anti-AF4 antibody NBP1-28705, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).Applications for AF4 Antibody
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Application Notes
Western Blot Not recommended.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: AF4
Alternate Names
AF-4, AF4/FMR2 family member 1, AF4/FMR2 family, member 1, AF4myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 2, ALL1-fused gene from chromosome 4 protein, FEL, MLLT2MGC134969, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 2, PBM1, pre-B-cell monocytic leukemia partner 1, Protein AF-4, Protein FEL, Proto-oncogene AF4
Entrez Gene IDs
4299 (Human)
Gene Symbol
AFF1
UniProt
Additional AF4 Products
Product Documents for AF4 Antibody
Product Specific Notices for AF4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...