Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation (Negative), Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all AF4 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human proto-oncogene AF4 using the numbering given in entry NP_005926.1 (GeneID 4299).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AF4 Antibody

Western Blot: AF4 Antibody [NBP1-28728]

Western Blot: AF4 Antibody [NBP1-28728]

Western Blot: AF4 Antibody [NBP1-28728] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-AF4 antibody NBP1-28728 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. AF4 was also immunoprecipitated by rabbit anti-AF4 antibody NBP1-28705, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Immunohistochemistry-Paraffin: AF4 Antibody [NBP1-28728]

Immunohistochemistry-Paraffin: AF4 Antibody [NBP1-28728]

Immunohistochemistry-Paraffin: AF4 Antibody [NBP1-28728] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- AF4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for AF4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Western Blot

1:2000-1:10000
Application Notes
Not recommended for immunoprecipitation. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: AF4

AF4 is a member of the AF4/LAF4/FMR2 (ALF) family of transcription cofactors. It has been identified as part of a translocation found in patients with acute leukemia involving MLL/HRX. AF4 functions as a cofactor of transcriptional elongation and chromatin remodeling.

Alternate Names

AF-4, AF4/FMR2 family member 1, AF4/FMR2 family, member 1, AF4myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 2, ALL1-fused gene from chromosome 4 protein, FEL, MLLT2MGC134969, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 2, PBM1, pre-B-cell monocytic leukemia partner 1, Protein AF-4, Protein FEL, Proto-oncogene AF4

Entrez Gene IDs

4299 (Human)

Gene Symbol

AFF1

UniProt

P51825 (Human)

Additional AF4 Products

Product Documents for AF4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for AF4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.