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AF4 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03250

Novus Biologicals, part of Bio-Techne
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NBP3-03250-100ul
NBP3-03250-20ul

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1124-1218 of human AF4 (NP_001160165.1). SQSSAGSVGSSGVAATISTPVTIQNMTSSYVTITSHVLTAFDLWEQAEALTRKNKEFFARLSTNVCTLALNSSLVDLVHYTRQGFQQLQELTKTP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AF4 Antibody - BSA Free

Western Blot: AF4 AntibodyBSA Free [NBP3-03250]

Western Blot: AF4 AntibodyBSA Free [NBP3-03250]

Western Blot: AF4 Antibody [NBP3-03250] - Analysis of extracts of various cell lines, using AF4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.
Immunocytochemistry/ Immunofluorescence: AF4 Antibody - BSA Free [NBP3-03250]

Immunocytochemistry/ Immunofluorescence: AF4 Antibody - BSA Free [NBP3-03250]

Immunocytochemistry/Immunofluorescence: AF4 Antibody [NBP3-03250] - Analysis of U2OS cells using AF4 antibody .

Applications for AF4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AF4

AF4 is a member of the AF4/LAF4/FMR2 (ALF) family of transcription cofactors. It has been identified as part of a translocation found in patients with acute leukemia involving MLL/HRX. AF4 functions as a cofactor of transcriptional elongation and chromatin remodeling.

Alternate Names

AF-4, AF4/FMR2 family member 1, AF4/FMR2 family, member 1, AF4myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 2, ALL1-fused gene from chromosome 4 protein, FEL, MLLT2MGC134969, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 2, PBM1, pre-B-cell monocytic leukemia partner 1, Protein AF-4, Protein FEL, Proto-oncogene AF4

Gene Symbol

AFF1

Additional AF4 Products

Product Documents for AF4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AF4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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