AFAP1L2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06316

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all AFAP1L2 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human AFAP1L2 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for AFAP1L2 Antibody

Western Blot: AFAP1L2 Antibody [NBP3-06316]

Western Blot: AFAP1L2 Antibody [NBP3-06316]

Western Blot: AFAP1L2 Antibody [NBP3-06316] - Anti-AFAP1L2 rabbit polyclonal antibody at 1:500 dilutionLane A: ACHN Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size: 91 kDa
Immunocytochemistry/ Immunofluorescence: AFAP1L2 Antibody [NBP3-06316]

Immunocytochemistry/ Immunofluorescence: AFAP1L2 Antibody [NBP3-06316]

Immunocytochemistry/Immunofluorescence: AFAP1L2 Antibody [NBP3-06316] - Immunofluorescence staining of AFAP1L2 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human AFAP1L2 polyclonal antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and cell membrane.
Immunoprecipitation: AFAP1L2 Antibody [NBP3-06316]

Immunoprecipitation: AFAP1L2 Antibody [NBP3-06316]

Immunoprecipitation: AFAP1L2 Antibody [NBP3-06316] - AFAP1L2 was immunoprecipitated using:Lane A: 0.5 mg U-251 MG Whole Cell Lysate4 uL anti-AFAP1L2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody:Anti-AFAP1L2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 91 kDaObserved band size :100 kDa

Applications for AFAP1L2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

1-5ul/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AFAP1L2

AFAP1L2 may play a role in a signaling cascade by enhancing the kinase activity of SRC. Contributes to SRC-regulatedtranscription activation

Alternate Names

actin filament associated protein 1-like 2, actin filament-associated protein 1-like 2, AFAP1-like protein 2, FLJ14564, KIAA1914Em:AC005383.4, XB130CTB-1144G6.4

Gene Symbol

AFAP1L2

Additional AFAP1L2 Products

Product Documents for AFAP1L2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AFAP1L2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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