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AFTPH Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38227

Novus Biologicals, part of Bio-Techne
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NBP2-38227
NBP2-38227-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: DSVPNIQDDCNGFQDSDDFADFSSAGPSQVVDWNAFEDEQKDSCSWAAFGDQQATESHHRKEAWQSHRTDENIDTPGTPKTHSVP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AFTPH Antibody

Western Blot: AFTPH Antibody [NBP2-38227]

Western Blot: AFTPH Antibody [NBP2-38227]

Western Blot: AFTPH Antibody [NBP2-38227] - Analysis in control (vector only transfected HEK293T lysate) and AFTPH over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: AFTPH Antibody [NBP2-38227]

Immunocytochemistry/ Immunofluorescence: AFTPH Antibody [NBP2-38227]

Immunocytochemistry/Immunofluorescence: AFTPH Antibody [NBP2-38227] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol, the Golgi apparatus & vesicles.
Immunohistochemistry-Paraffin: AFTPH Antibody [NBP2-38227]

Immunohistochemistry-Paraffin: AFTPH Antibody [NBP2-38227]

Immunohistochemistry-Paraffin: AFTPH Antibody [NBP2-38227] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.

Applications for AFTPH Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AFTPH

AFTPH may play a role in membrane trafficking

Alternate Names

AFTH, aftiphilin, aftiphilin protein, FLJ20080, FLJ23793, MGC33965, Nbla10388

Gene Symbol

AFTPH

UniProt

Additional AFTPH Products

Product Documents for AFTPH Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AFTPH Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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