AG-2/AGR2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05936

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all AG-2/AGR2 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an N-terminal portion of mouse AGR2 (within residues 1-50). [Swiss-Prot# O88312]

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AG-2/AGR2 Antibody - BSA Free

Western Blot: AG-2/AGR2 AntibodyBSA Free [NBP1-05936]

Western Blot: AG-2/AGR2 AntibodyBSA Free [NBP1-05936]

Western Blot: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - Human stomach lysate.
Immunocytochemistry/ Immunofluorescence: AG-2/AGR2 Antibody - BSA Free [NBP1-05936]

Immunocytochemistry/ Immunofluorescence: AG-2/AGR2 Antibody - BSA Free [NBP1-05936]

Immunocytochemistry/Immunofluorescence: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - AGR2 antibody was tested in MCF7 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NBP1-05936]

Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NBP1-05936]

Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - Representative immunohistological analysis of CD163 (scavenger receptor present in macrophages), AGR2, and TMED2 in non-inflamed colonic biopsies from healthy controls and patients with active or quiescent CD. Image collected and cropped by CiteAb from the following publication (https://onlinelibrary.wiley.com/doi/10.15252/emmm.201810120) licensed under a CC-BY license.
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Applications for AG-2/AGR2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100-1:300

Immunohistochemistry-Paraffin

1:100-1:300

Western Blot

1.0 ug/ml
Application Notes
This AGR2 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections, and Western blot analysis where a band can be seen at ~19.9 kDa. In ICC/IF cytoplasmic staining is observed. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AG-2/AGR2

Originally discovered in Xenopus laevis wherein it plays a role during early neural development, AGR2 (anterior gradient protein 2 homolog) is now known as a member of protein disulfide isomerase (PDI) family of ER-resident proteins which is expressed in a variety of normal as well as transformed epithelial tissues. AGR2 has been implicated in the folding of proteins as a chaperone protein. It interacts with LYPD3, DAG1 (alphaDAG1) and MUC2 (disulfide-linked), and is essential for MUC2 translation as well as secretion. AGR2 plays a key role in the production of mucus by intestinal cells and has also been suggested to be a proto-oncogene that may involve in cellular migration, differentiation and growth. Differential expression of AGR2 was first found in estrogen receptor (ER)-positive breast cancer cell lines and subsequently, elevated AGR2 levels have been seen in subsets of other cancers also wherein its upregulation enhances classical hallmarks of cancer such as metastasis, invasion, colony formation, and proliferation.

Long Name

Anterior Gradient 2 Homolog

Alternate Names

AG2, AGR2, GOB-4, HPC8, PDIA17

Gene Symbol

AGR2

Additional AG-2/AGR2 Products

Product Documents for AG-2/AGR2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AG-2/AGR2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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