AGER Antibody (2A11) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11999

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-11999-0.025mg
NBP3-11999-0.2mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Surface Plasmon Resonance, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 2A11

Format

Azide and BSA Free

Concentration

1 mg/ml

View all RAGE/AGER Antibodies »

Product Summary for AGER Antibody (2A11) - Chimeric - Azide and BSA Free

Immunogen

Human AGER protein.

Specificity

2A11 is specific for the V-domain of human advanced glycosylation end product-specific receptor. It recognises both the glycosylated and non-glycosylated forms.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for AGER Antibody (2A11) - Chimeric - Azide and BSA Free

Western Blot: AGER Antibody (2A11)ChimericAzide and BSA Free [NBP3-11999]

Western Blot: AGER Antibody (2A11)ChimericAzide and BSA Free [NBP3-11999]

Western Blot: AGER Antibody (2A11) - Chimeric [NBP3-11999] - Human lung lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric rabbit IgG version of 2A11 (NBP3-11999) at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: AGER Antibody (2A11) - Chimeric - Azide and BSA Free [NBP3-11999]

Immunocytochemistry/ Immunofluorescence: AGER Antibody (2A11) - Chimeric - Azide and BSA Free [NBP3-11999]

Immunocytochemistry/Immunofluorescence: AGER Antibody (2A11) - Chimeric [NBP3-11999] - Immunofluorescence analysis of paraformaldehyde fixed A549 cells stained with the chimeric rabbit IgG version of 2A11 (NBP3-11999) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11999, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor(R) 488 secondary antibody.
Flow Cytometry: AGER Antibody (2A11) - Chimeric - Azide and BSA Free [NBP3-11999]

Flow Cytometry: AGER Antibody (2A11) - Chimeric - Azide and BSA Free [NBP3-11999]

Flow Cytometry: AGER Antibody (2A11) - Chimeric [NBP3-11999] - A549 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, shaded line) or the rabbit IgG version of 2A11 (NBP3-11999, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Applications for AGER Antibody (2A11) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Surface Plasmon Resonance

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
ELISA confirmed the specificity of 2A11 for the V-domain of AGER, with a binding affinity (Kd) of 7.6 nM. ELISA was also used to confirm that the binding of 2A11 to AGER is glycosylation independent. In SPR experiments the Kd of 2A11 binding to AGER was determined as 0.38 nM. Flow cytometry and immunochemistry were used to visualise the binding of 2A11 to HEK-AGER cells. This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: RAGE/AGER

AGER encodes a member of the immunoglobulin superfamily of cell surface molecules. It is a receptor for various molecules, including the amyloidogenic form of serum amyloid A, amyloid-beta protein, members of the S100/calgranulin superfamily and advanced glycation end products. The gene lies within the major histocompatibility complex (MHC) class III region on chromosome 6. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]

Long Name

Receptor for Advanced Glycation End Products

Alternate Names

AGER, SCARJ1

Gene Symbol

AGER

Additional RAGE/AGER Products

Product Documents for AGER Antibody (2A11) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for AGER Antibody (2A11) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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