AGL Antibody (PSH04-56)

Immunohistochemistry: AGL Antibody (PSH04-56) [NBP3-32009]

Immunohistochemistry: AGL Antibody (PSH04-56) [NBP3-32009] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-AGL antibody (NBP3-32009) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32009) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: AGL Antibody (PSH04-56) [NBP3-32009]

Western Blot: AGL Antibody (PSH04-56) [NBP3-32009] - Western blot analysis of AGL on different lysates with Rabbit anti-AGL antibody (NBP3-32009) at 1/1,000 dilution.

Lane 1: K-562 cell lysate (20 ug/Lane)
Lane 2: HEK-293 cell lysate (20 ug/Lane)
Lane 3: Mouse skeletal muscle tissue lysate (10 ug/Lane)
Lane 4: Rat skeletal muscle tissue lysate (10 ug/Lane)

Lysates/proteins at 10 ug/Lane.

Predicted band size: 175 kDa
Observed band size: 150 kDa

Exposure time: Lane 1-2: 3 minutes; Lane 3-4: 20 seconds; ECL;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32009) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.