Ago2/eIF2C2 Antibody (036)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90675

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP2-90675-100ul
NBP2-90675-50ul
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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 036

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Ago2/eIF2C2 Antibodies »

Product Summary for Ago2/eIF2C2 Antibody (036)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Ago2/eIF2C2 (Uniprot#: Q8CJG0; Met1-Ala860).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Ago2/eIF2C2 Antibody (036)

Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (036) [NBP2-90675]

Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (036) [NBP2-90675]

Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (036) [NBP2-90675] - Immunochemical staining of AGO2 in human brain with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (036) [NBP2-90675]

Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (036) [NBP2-90675]

Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (036) [NBP2-90675] - Immunochemical staining of AGO2 in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).
Immunoprecipitation: Ago2/eIF2C2 Antibody (036) [NBP2-90675]

Immunoprecipitation: Ago2/eIF2C2 Antibody (036) [NBP2-90675]

Immunoprecipitation: Ago2/eIF2C2 Antibody (036) [NBP2-90675] - AGO2 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg Raw264.7 Whole Cell Lysate2 uL anti-Mouse AGO2 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse AGO2 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 100 kDaObserved band size: 100 kDa
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Applications for Ago2/eIF2C2 Antibody (036)

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

1-4 uL/mg of lysate
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ago2/eIF2C2

Ago2 / eIF2C2 encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.

Alternate Names

AGO2argonaute 2, Argonaute2, EC 3.1.26.n1, EC 3.1.26.n2, eIF2C 2, eIF-2C 2, Eukaryotic translation initiation factor 2C 2, eukaryotic translation initiation factor 2C, 2, hAgo2, MGC3183, PAZ Piwi domain protein, PPD, protein argonaute-2, Protein slicer, Q10

Gene Symbol

AGO2

Additional Ago2/eIF2C2 Products

Product Documents for Ago2/eIF2C2 Antibody (036)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ago2/eIF2C2 Antibody (036)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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