Ago2/eIF2C2 Antibody (10S10L5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15873
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10S10L5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 350-450 of human Ago2/eIF2C2 (Q9UKV8). QRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKV
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Ago2/eIF2C2 Antibody (10S10L5)
![Western Blot: Ago2/eIF2C2 Antibody (10S10L5) [NBP3-15873]](https://resources.bio-techne.com/images/products/Ago2-eIF2C2-Antibody-10S10L5-Western-Blot-NBP3-15873-img0004.jpg "Western Blot: Ago2/eIF2C2 Antibody (10S10L5) [NBP3-15873]")
Western Blot: Ago2/eIF2C2 Antibody (10S10L5) [NBP3-15873]
Western Blot: Ago2/eIF2C2 Antibody (10S10L5) [NBP3-15873] - Western blot analysis of extracts of various cell lines, using Ago2/eIF2C2 antibody (NBP3-15873) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (10S10L5) [NBP3-15873] -
Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (10S10L5) [NBP3-15873] - Rat brain using Argonaute-2 Rabbit mAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (10S10L5) [NBP3-15873] -
Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (10S10L5) [NBP3-15873] - Mouse liver tissue using Argonaute-2 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.Applications for Ago2/eIF2C2 Antibody (10S10L5)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Ago2/eIF2C2
Alternate Names
AGO2argonaute 2, Argonaute2, EC 3.1.26.n1, EC 3.1.26.n2, eIF2C 2, eIF-2C 2, Eukaryotic translation initiation factor 2C 2, eukaryotic translation initiation factor 2C, 2, hAgo2, MGC3183, PAZ Piwi domain protein, PPD, protein argonaute-2, Protein slicer, Q10
Gene Symbol
AGO2
Additional Ago2/eIF2C2 Products
Product Documents for Ago2/eIF2C2 Antibody (10S10L5)
Product Specific Notices for Ago2/eIF2C2 Antibody (10S10L5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...