Ago2/eIF2C2 Antibody (11A9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23228
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Bovine
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 11A9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Ago2/eIF2C2 Antibody (11A9)
Immunogen
Clone 11A9 was generated from an Ago2/eIF2C2 synthetic peptide (coupled to KLH) corresponding to amino acids in the N-terminus of human Ago2/eIF2C2. This sequence has low homology to other argonaute family members. [UniProt# Q9UKV8]
Specificity
The antibody detects a 97 kDa protein corresponding to the apparent molecular mass ofAgo2/eIF2C2 on SDS-PAGE immunoblots of several human cell lines, A431, HeLa, MCF-7, and K562. The antibody does not detect rat or mouse Ago2/eIF2C2, but will detect bovine Ago2/eIF2C2.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
Scientific Data Images for Ago2/eIF2C2 Antibody (11A9)
Western Blot: Ago2/eIF2C2 Antibody (11A9) [NBP3-23228] -
Western Blot: Ago2/eIF2C2 Antibody (11A9) [NBP3-23228] - Western blot analysis of human A431 cells treated with EGF (100 ng/ml for 60 min.) (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rat monoclonal anti-Ago2/eIF2C2 (NBP3-23228) (lanes 1 & 2) and rabbit polyclonal anti-Ago2/eIF2C2 (Tyr-393) phospho-specific antibody (lanes 3 & 4).Applications for Ago2/eIF2C2 Antibody (11A9)
Application
Recommended Usage
Western Blot
1:250
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Ago2/eIF2C2
Alternate Names
AGO2argonaute 2, Argonaute2, EC 3.1.26.n1, EC 3.1.26.n2, eIF2C 2, eIF-2C 2, Eukaryotic translation initiation factor 2C 2, eukaryotic translation initiation factor 2C, 2, hAgo2, MGC3183, PAZ Piwi domain protein, PPD, protein argonaute-2, Protein slicer, Q10
Gene Symbol
AGO2
Additional Ago2/eIF2C2 Products
Product Documents for Ago2/eIF2C2 Antibody (11A9)
Product Specific Notices for Ago2/eIF2C2 Antibody (11A9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...