Ago2/eIF2C2 Antibody (JF0992)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67121

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-67121
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JF0992

Concentration

1 mg/ml

View all Ago2/eIF2C2 Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Ago2/eIF2C2 aa 361-404 / 859. (SwissProt: Q9UKV8 Human; SwissProt: Q8CJG0 Mouse; SwissProt: Q9QZ81 Rat)

Localization

Cytoplasm, Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ago2/eIF2C2 Antibody (JF0992)

Western Blot: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121]

Western Blot: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121]

Western Blot: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Analysis of Argonaute 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysateLane 2: Hela cell lysate
Immunocytochemistry/ Immunofluorescence: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121]

Immunocytochemistry/ Immunofluorescence: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121]

Immunocytochemistry/Immunofluorescence: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Staining Argonaute 2 in AGS cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121]

Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121]

Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-Argo2/elF2C2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Applications for Ago2/eIF2C2 Antibody (JF0992)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:4000

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ago2/eIF2C2

Ago2 / eIF2C2 encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.

Alternate Names

AGO2argonaute 2, Argonaute2, EC 3.1.26.n1, EC 3.1.26.n2, eIF2C 2, eIF-2C 2, Eukaryotic translation initiation factor 2C 2, eukaryotic translation initiation factor 2C, 2, hAgo2, MGC3183, PAZ Piwi domain protein, PPD, protein argonaute-2, Protein slicer, Q10

Gene Symbol

AGO2

Additional Ago2/eIF2C2 Products

Product Documents for Ago2/eIF2C2 Antibody (JF0992)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Ago2/eIF2C2 Antibody (JF0992)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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