Ago2/eIF2C2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13024

Novus Biologicals, part of Bio-Techne
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NBP3-13024
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (100%), Mouse (100%), Porcine (100%), Rabbit (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Ago2/eIF2C2 Antibodies »

Product Summary for Ago2/eIF2C2 Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Ago2/eIF2C2. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Ago2/eIF2C2 Antibody

Western Blot: Ago2/eIF2C2 Antibody [NBP3-13024]

Western Blot: Ago2/eIF2C2 Antibody [NBP3-13024]

Western Blot: Ago2/eIF2C2 Antibody [NBP3-13024] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AGO2 antibody (NBP3-13024) diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Ago2/eIF2C2 Antibody [NBP3-13024]

Immunocytochemistry/ Immunofluorescence: Ago2/eIF2C2 Antibody [NBP3-13024]

Immunocytochemistry/Immunofluorescence: Ago2/eIF2C2 Antibody [NBP3-13024] - AGO2 antibody detects AGO2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AGO2 protein stained by AGO2 antibody (NBP3-13024) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody [NBP3-13024]

Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody [NBP3-13024]

Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody [NBP3-13024] - Ago2/eIF2C2 antibody detects Ago2/eIF2C2 protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Ago2/eIF2C2 antibody (NBP3-13024) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for Ago2/eIF2C2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ago2/eIF2C2

Ago2 / eIF2C2 encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.

Alternate Names

AGO2argonaute 2, Argonaute2, EC 3.1.26.n1, EC 3.1.26.n2, eIF2C 2, eIF-2C 2, Eukaryotic translation initiation factor 2C 2, eukaryotic translation initiation factor 2C, 2, hAgo2, MGC3183, PAZ Piwi domain protein, PPD, protein argonaute-2, Protein slicer, Q10

Gene Symbol

AGO2

Additional Ago2/eIF2C2 Products

Product Documents for Ago2/eIF2C2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Ago2/eIF2C2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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