Ago2/eIF2C2 [p Ser387] Antibody

Biological Validation

Validated:

Unconjugated

Polyclonal Rabbit IgG

Please see the vial label for concentration. If unlisted please contact technical services.

Phospho-Ago2/eIF2C2 (Ser-387) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 387 in human Ago2/eIF2C2. This peptide sequence is highly conserved in mouse, rat, chick, and fish Ago2/eIF2C2. The site is also conserved in Ago1 and Ago4, but the homology surrounding the site is low between Ago2 and these two family members. [UniProt# Q9UKV8]

p Ser387

This antibody was cross-absorbed to unphospho-Ago2/eIF2C2 (Ser-387), before affinity purification using phospho-Ago2/eIF2C2 (Ser-387) peptide (without carrier). The antibody detects a 97 kDa protein corresponding to the apparent molecular mass of phosphorylated Ago2 on SDS-PAGE immunoblots of human A431 cells treated with Calyculin A and mouse recombinant Ago2/eIF2C2 full length protein. This reactivity is not observed after lambda phosphatase dephosphorylation.

Polyclonal

Rabbit

IgG

97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Stable for at least 1 year at -20°C.

Antigen Affinity-purified

PBS, 1 mg/ml BSA and 50% glycerol

0.05% Sodium Azide

Please see the vial label for concentration. If unlisted please contact technical services.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at -20C.