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AGPAT5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83767

Novus Biologicals, part of Bio-Techne
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NBP1-83767

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ANHQSTVDWIVADILAIRQNALGHVRYVLKEGLKWLPLYGCYFAQHGGIYVKRSAKFNEKEMRNKLQSYVDAGTPMYLVIFPEGTRYNPEQTKVLSASQAFAAQRGLAVLKHVLTPRIK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (85%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AGPAT5 Antibody

Western Blot: AGPAT5 Antibody [NBP1-83767]

Western Blot: AGPAT5 Antibody [NBP1-83767]

Western Blot: AGPAT5 Antibody [NBP1-83767] - Analysis in control (vector only transfected HEK293T lysate) and AGPAT5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: AGPAT5 Antibody [NBP1-83767]

Immunohistochemistry-Paraffin: AGPAT5 Antibody [NBP1-83767]

Immunohistochemistry-Paraffin: AGPAT5 Antibody [NBP1-83767] - Staining of human corpus, uterine shows strong cytoplasmic positivity in glandular cells.

Applications for AGPAT5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AGPAT5

AGPAT5 encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. This integral membrane protein converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. [provided by RefSeq]

Alternate Names

1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acidacyltransferase, epsilon), 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon, 1-AGPAT 5,1-acylglycerol-3-phosphate O-acyltransferase 5, 1AGPAT5,1-AGP acyltransferase 5, EC 2.3.1.51, FLJ11210, LPAATE, LPAAT-e, LPAAT-epsilon, Lysophosphatidic acid acyltransferase epsilon

Entrez Gene IDs

55326 (Human)

Gene Symbol

AGPAT5

UniProt

Additional AGPAT5 Products

Product Documents for AGPAT5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AGPAT5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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