Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all AHI1 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 1146 and 1196 of human Abelson Helper Integration site 1 using the numbering given in entry NP_001128302.1 (GeneID 54806).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AHI1 Antibody

Western Blot: AHI1 Antibody [NBP1-78206]

Western Blot: AHI1 Antibody [NBP1-78206]

Western Blot: AHI1 Antibody [NBP1-78206] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and Jurkat (J; 50 mcg) cells. Affinity purified rabbit anti-AHI1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. AHI1 was also immunoprecipitated by rabbit anti-AHI1 antibody NBP1-78205, which recognizes an upstream epitope.

Applications for AHI1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 mcg/mg

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: AHI1

Jouberin (AHI1), encoding the Jouberin protein, is an alternatively spliced signaling molecule that contains seven Trp-Asp (WD) repeats, an SH3 domain, and numerous SH3-binding sites. The gene is expressed strongly in embryonic hindbrain and forebrain, and AHI1 may be required for both cerebellar and cortical development in humans. Defects in AHI1 are the cause of Joubert syndrome 3, an autosomal recessive brain congenital malformation. The exact function of the AHI1 gene is not yet known.

Alternate Names

Abelson helper integration site, Abelson helper integration site 1, Abelson helper integration site 1 protein homolog, AHI-1, contatins SH3 and WD40 domains, dJ71N10.1, DKFZp686J1653, FLJ14023, FLJ20069, JBTS3, jouberin, ORF1

Gene Symbol

AHI1

UniProt

NP_001128302.1 (Human)

Additional AHI1 Products

Product Documents for AHI1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for AHI1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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