Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # 25F2.D10

Format

Azide and BSA Free

Concentration

1.59 mg/ml

View all AHSA1 Antibodies »

Product Specifications

Immunogen

Mouse Aha1

Localization

Cytoplasm , Endoplasmic Reticulum

Specificity

Detects approx 38kDa. Can run up to 45kDa on SDS Page.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for AHSA1 Antibody (25F2.D10) - Azide and BSA Free

Western Blot: AHSA1 Antibody (25F2.D10)Azide and BSA Free [NBP2-12884]

Western Blot: AHSA1 Antibody (25F2.D10)Azide and BSA Free [NBP2-12884]

Western Blot: AHSA1 Antibody (25F2.D10) - Azide and BSA Free [NBP2-12884] - Western Blot analysis of Human Cell lysates showing detection of AHSA1 protein using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10 (NBP2-12884). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-AHSA1 Monoclonal Antibody (NBP2-12884) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: AHSA1 Antibody (25F2.D10) - Azide and BSA Free [NBP2-12884]

Immunocytochemistry/ Immunofluorescence: AHSA1 Antibody (25F2.D10) - Azide and BSA Free [NBP2-12884]

Immunocytochemistry/Immunofluorescence: AHSA1 Antibody (25F2.D10) - Azide and BSA Free [NBP2-12884] - Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Rat (green).
Immunohistochemistry: AHSA1 Antibody (25F2.D10) - Azide and BSA Free [NBP2-12884]

Immunohistochemistry: AHSA1 Antibody (25F2.D10) - Azide and BSA Free [NBP2-12884]

Immunohistochemistry: AHSA1 Antibody (25F2.D10) - Azide and BSA Free [NBP2-12884] - Immunohistochemistry analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10 (NBP2-12884). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (NBP2-12884) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:50 for 1 hour at RT. Localization: Uppermost epidermis staining, and muscle.
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Applications for AHSA1 Antibody (25F2.D10) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:1000

Western Blot

1:1000
Application Notes
1 ug/ml of AHA1 Antibody was sufficient for detection of Aha1 in 10 ug of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

20 mM Potassium Phosphate, 0.15 M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.59 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AHSA1

1 ug/mL was sufficient for detection of Aha1 in 10ug of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG: HRP as the secondary antibody.

Alternate Names

activator of 90 kDa heat shock protein ATPase homolog 1, AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast), C14orf3, chromosome 14 open reading frame 3, p38AHA1

Gene Symbol

AHSA1

Additional AHSA1 Products

Product Documents for AHSA1 Antibody (25F2.D10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AHSA1 Antibody (25F2.D10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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